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114391

Sigma-Aldrich

Bromophenol Blue

ACS reagent

Synonyme(s) :

3′,3″,5′,5″-Tetrabromophenolsulfonphthalein, Bromphenol Blue Sultone Form

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About This Item

Formule empirique (notation de Hill):
C19H10Br4O5S
Numéro CAS:
115-39-9
Poids moléculaire :
669.96
Numéro Beilstein :
61698
Numéro CE :
204-086-2
Numéro MDL:
MFCD00005875
Code UNSPSC :
12171500
ID de substance PubChem :
24847169
Nomenclature NACRES :
NA.47

Qualité

ACS reagent

Niveau de qualité

200

Forme

powder

Technique(s)

titration: suitable

visual transition interval

3.0-4.6, yellow to blue

Pf

273 °C (lit.)

Solubilité

ethanol: 0.1%

Application(s)

diagnostic assay manufacturing
hematology
histology

Température de stockage

room temp

Chaîne SMILES 

Oc1c(Br)cc(cc1Br)C2(OS(=O)(=O)c3ccccc23)c4cc(Br)c(O)c(Br)c4

InChI

1S/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H

Clé InChI

UDSAIICHUKSCKT-UHFFFAOYSA-N

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Catégories apparentées

Description générale

Bromophenol blue is present as a sulfonated hydroxyquinone in acidic aqueous solutions. It is also known as tetrabromophenolsulfonphthalein.Bromophenol Blue is a pH indicator and a dye appearing as a strong blue color. It has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Application

Bromphenol Blue is used as a tracking dye in DNA, RNA (agarose), and protein (polyacrylamide) gel electrophoresis. It has been used in the preparation of protein samples for western blotting analysis. Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels. It has also been known for its use as a vital stain to probe the blood-brain barrier.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
SHBR7468
BCCM1010
SHBR1340
BCCJ9036
BCCK2682

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