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10102121001

Roche

Adenosine Deaminase (ADA)

from calf intestine

Synonyme(s) :

ADA, Adenosine Deaminase

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About This Item

Numéro de classification (Commission des enzymes):
Code UNSPSC :
12352204

Source biologique

bovine intestine

Niveau de qualité

Forme

suspension

Activité spécifique

~200 units/mg protein (At 25 °C with adenosine as the substrate.)

Conditionnement

pkg of 1 mL (10 mg)

Fabricant/nom de marque

Roche

Technique(s)

activity assay: suitable

Couleur

white

pH optimal

7.0-7.4

Solubilité

water: miscible

Adéquation

suitable for chemiluminescence

Numéro d'accès NCBI

Numéro d'accès UniProt

Application(s)

life science and biopharma

Activité étrangère

AMP-deaminase <0.01%
AP <0.01% (with pre incubation)
Guanase <0.01%
NP <0.01%

Température de stockage

2-8°C

Informations sur le gène

bovine ... ADA(280712)

Description générale

Adenosine aminohydrolase.
Adenosine deaminase (ADA) is a zinc metalloenzyme.

Application

Chemiluminescent adenosine assay.
Deamination of adenosine analogs to the corresponding inosine analogs
Adenosine deaminase (ADA) has been used in lipolysis assay along with other reagents.

Actions biochimiques/physiologiques

Adenosine deaminase (ADA) is involved in the deamination of adenosine to inosine and 2′ deoxyadenosine to 2′-deoxyinosine.

Qualité

Contaminants: <0.01% alkaline phosphatase, AMP-deaminase, guanase, and nucleoside phosphorylase each

Forme physique

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6. Small particles are normal. Before using stir up the precipitate and proceed.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

No data available

Point d'éclair (°C)

No data available


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Consulter la Bibliothèque de documents

Mi-Jeong Lee et al.
Methods in enzymology, 538, 49-65 (2014-02-18)
Obesity is reaching epidemic proportions so there is growing interest in the mechanisms that regulates adipose tissue development and function. Although murine adipose cell lines are useful for many mechanistic studies, primary human adipose stromal cells (ASCs), which can be
Cultured astrocytes do not release adenosine during hypoxic conditions.
Fujita T, et al.
Journal of Cerebral Blood Flow and Metabolism, 32(1), e1-e7 (2012)
Adenosine deaminase from calf intestinal mucosa.
C A ZITTLE
The Journal of biological chemistry, 166(2), 499-503 (1946-12-01)
D J Porter et al.
The Journal of biological chemistry, 268(4), 2480-2485 (1993-02-05)
The protein fluorescence of adenosine deaminase (ADA) was perturbed during the deamination of adenosine and four analogues of adenosine. The kinetics for the approach to the steady-state during turnover were monitored by fluorescence changes associated with formation of enzymatic intermediates.
Sonia Muñoz-López et al.
Frontiers in endocrinology, 13, 1007801-1007801 (2022-11-22)
Despite great efforts, effective treatment against cancer has not yet been found. However, natural compounds such as the polyphenol resveratrol have emerged as promising preventive agent in cancer therapy. The mode of action of resveratrol is still poorly understood, but

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