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PHCC360KIT

Millipore

Compteur de cellules automatisé portatif Scepter 3.0

Scepter 3.0 Handheld Automated Cell Counter

Rapid cell counts in a handheld easy to use format, includes pkg of 60 μm Scepter 3.0 Cell Counter Sensors

Synonyme(s) :

Cell counting instrument

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1 EACH
4 170,00 €

4 170,00 €


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4 170,00 €

About This Item

Code UNSPSC :
41131501
Nomenclature NACRES :
NC.56

4 170,00 €


Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.

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Fabricant/nom de marque

Scepter

Technique(s)

cell counting: suitable

Taille des particules

6-36 μm

Compatibilité

for use with cells sized 4.0–25.0 μm

Conditions d'expédition

ambient

Description générale

Le compteur de cellules automatisé portatif Scepter 3.0 permet d'effectuer un comptage cellulaire rapide dans un format facile d'utilisation que l'on tient à la main. Ce système est uniquement destiné à la recherche ; Scepter 3.0 a été testé sur des types de cellules représentatifs des usages actuels. Après avoir préparé une dilution de la culture de cellules à analyser, les instructions à l'écran guident l'utilisateur au travers du processus d'échantillonnage. Moins de 30 secondes après avoir aspiré un échantillon dans sa sonde, le compteur de cellules Scepter affiche à l'écran les résultats suivants :

  • Concentration de cellules
  • Taille moyenne des cellules
  • Volume cellulaire moyen
  • Histogramme de la répartition de la taille ou du volume
Le dispositif peut conserver les données de 999 comptages. Celles-ci peuvent être téléchargées sur l'ordinateur de l'utilisateur puis rechargées dans l'appareil pour des analyses plus poussées.

Le compteur de cellules Scepter 3.0 est compatible avec les sondes pour compteur de cellules Scepter 3.0 40 µM et 60 µM. Ce kit (réf. PHCC360KIT) contient un paquet gratuit de 50 sondes 60 µM.

Composants

  • 1 compteur de cellules automatisé portatif Scepter 3.0
  • 1 fiole de billes de test
  • 1 station de charge
  • 50 sondes (60 µM)

Autres remarques

Le compteur de cellules Scepter 3.0 n'est pas compatible avec les sondes des générations précédentes ; il n'est compatible qu'avec les sondes pour compteur de cellules Scepter 3.0 figurant dans la section Produits apparentés.

Code de la classe de stockage

10-13 - German Storage Class 10 to 13

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Articles

Data for validated human cell lines using the Millicell® DCI Digital Cell Imager for confluency determination and estimated cell counts.

Find answers to frequently asked questions about the Millicell® ERS 3.0, from probe depth adjustment to instrument maintenance and troubleshooting.

Learn how Sterivex™ pressure-driven sterile filters can be used for large-volume sample processing in environmental research and testing.

The Scepter™ 3.0 Cell Counter, when used in conjunction with a 40 μm aperture sensor, can accurately count and precisely size a wide range of cell types and particles. This application note demonstrates two examples of experiments using the instrument’s sensitive size-discriminating capacity for rapid qualitative assessment of individual cell populations.

Protocoles

Cell culture protocol for passaging and splitting adherent cell lines using trypsin EDTA. Free ECACC handbook download.

Contenu apparenté

Rationalisez la capture et l'analyse des données de mesure de la TEER grâce à des améliorations conviviales, notamment une interface tactile intuitive, une sonde autonome dans les puits et l'enregistrement automatique des données.

Monitor your cell cultures with ease with the Millicell® DCI Digital Cell Imager. From confluency to cell count and morphology, the Millicell® DCI automates cell culture measurements for quicker, more consistent culturing.

Scepter™ Cell Counter is a portable device which brings consistency in cell counting right to the culture hood in less than 30 seconds.

Scepter™ counting is 7 to 10 times faster than hemocytometry-and also faster than other automated counters. Learn more about how this amazing technology works

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Questions

1–3 of 3 Questions  
  1. What is the maximum size of cell clumps that can pass through the Scepter 3.0? Also, what size screen is used to break up the cell clump aggregates in both Scepter 2.0 and 3.0?

    1 answer
    1. The maximum size of cell clumps that can pass through the Scepter 3.0 is determined by the aperture limits, which are 40um and 60um for sensors 40 and 60, respectively. Unlike the Scepter 2.0, the Scepter 3.0 does not use a screen to break up cell clump aggregates; instead, it employs a set of pillars to achieve this function. In contrast, the Scepter 2.0 utilizes a 35um Nylon filter to break up cell clump aggregates.

      Helpful?

  2. Is this appropriate for size 15-23 μm cell? Does the Kit include also the charger? How many 60/40 μm sensors are included Which sensors can be used with this instrument? Which sensors are appropriate for cells size 15-23 μm and what is their price?

    1 answer
    1. Please see the answers to the following questions below:

      1. Is this appropriate for size 15-23 μm cells?
      Yes, the Scepter 3.0 is suitable. As listed on the product detail page in the DESCRIPTION section for use with cells sized 4.0–25.0 μm.

      2. Does the Kit include the charger?
      This product does include 60μM sensors and a charging station.

      3. How many 60/40 μm sensors are included
      As mentioned on the product detail page in the DESCRIPTION section under 'Components', it includes 50 Sensors.

      4. Which sensors can be used with this instrument?
      This product is only compatible with Scepter 3.0 Cell Counter Sensors shown in the Related Products on this page. Please see the link below to review additional information regarding the Scepter 3.0:
      https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/cell-counting-and-health-analysis/scepter-cell-counter

      5. Which sensors are appropriate for cells size 15-23 μm and what is their price?
      See question 1, this product is suitable for the cell size of 15-23 μm.

      Regarding pricing, Please navigate to the link https://www.sigmaaldrich.com/support/customer-support, and click on "Products Pricing & Availability" under the Products Section with all the required information so that a member of the Customer Service team can reach out to assist further. Thank you.

      Helpful?

  3. Does it require Trypan blue or another dye? Does it distinguish between live and dead cells?

    1 answer
    1. The Scepter is a particle counter, but it will not differentiate live from dead cells. The device can be used to gauge cell health on the basis of cell size. For example, in cell types where apoptotic cells are smaller in size compared to non-apoptotic cells. This shift in size can be quantified using the device option "Edit Histogram" to either gate around this population or exclude the population from the count.

      Please see the following manual below for more information:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/799/484/00110230w-rev1020-ms.pdf

      Helpful?

Reviews

Active Filters

  1. R. Moldova
    • Review 1
    • Votes 4
    3 out of 5 stars.

    Suggestion

    I could have been more careful when ordering the Scepter 3.0 components (Handheld Automated Cell Counter and Cell sensors), because without experience I ordered sensors not suitable for this type of reader. That is, instead of PHCC360500
    Cell Counter Sensors I ordered PHCC60500.
    No one raised any questions. Regrettable. I will have to incur additional expenses.
    I really hope to fix this mistake to be able to apply this device in practice.

    Helpful?

    1. Response from MilliporeSigma:

      Thank you for reaching out and sharing your review with us. We strive to provide the information customers need to make a confident purchase. Your feedback is extremely valuable in helping us to update the product information for this product to clarify which sensors are compatible with the Scepter 3.0.

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