MABS1164
Anti-Nucleobindin-2/Nestafin-1 Antibody, clone 3B3.1
clone 3B3.1, from mouse
Synonyme(s) :
Nucleobindin-2, Nestafin-1, DNA-binding protein NEFA, Prepronesfatin, Pronesfatin
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
3B3.1, monoclonal
Espèces réactives
human, mouse, rat
Technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
Isotype
IgG1κ
Numéro d'accès NCBI
Numéro d'accès UniProt
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
rat ... Nucb2(59295)
Description générale
Nucleobindin-2 (UniProt Q9JI85; also known as DNA-binding protein NEFA, Prepronesfatin) is encoded by the Nucb2 (also known as Nefa) gene (Gene ID 59295) in rat species. Nucleobindin-2 is initially translated as a 420-a.a. precursor protein. Cleavage of signal peptide (aa1-24) yields the 396-a.a. mature nucleobindin-2 with very high sequence homology among human, mouse, and rat species (more than 85%). Post-translational processing by prohormone convertases (PC 1/3 and PC 2) cleaves nucleobindin-2 into nesfatin-1, nesfatin-2, and nesfatin-3 (aa1–82, aa85–163, and aa166–396, respectively, numbering based on mature sequence). Nesfatin-1 is a well recognized circulating anorexigenic peptide, whose production increases post-prandially. Nucleobindin-2 contains two calcium-binding EF-hand domains separated by an acidic amino acid-rich region, and a leucine zipper. Nucleobindin-2 is known to be expressed in key hypothalamic nuclei and plays a role in energy homeostasis. Nucleobindin-2 is also expressed in various human peripheral tissues, including the stomach, pancreas, reproductive organs, and adipose tissues, suggesting roles in various metabolic functions. Nucleobindin-2 expression level is reported to be elevated in clear cell renal cell carcinoma (ccRCC), breast cancer, and prostate cancer (PCa) tissues.
Spécificité
Clone 3B3.1 reacts with pro-nucleobindin-2, mature nucleobindin-2, and nesfatin-1, but not nesfatin-2 or nesfatin-3.
Immunogène
Epitope: Nesfatin-1
GST-tagged recombinant rat Nestafin-1.
Application
Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected nucleobindin-2/nestafin-1 immunoreactivity in human colon cancer tissue, as well as in normal testis, tonsil, and pancreas tissue sections.
Research Category
Signaling
Signaling
Research Sub Category
Metabolic Hormones & Receptors
Metabolic Hormones & Receptors
This Anti-Nucleobindin-2 Antibody, clone 3B3.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of Nestafin-1.
Qualité
Evaluated by Western Blotting in mouse liver tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected nucleobindin-2 in 10 µg of mouse liver tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected nucleobindin-2 in 10 µg of mouse liver tissue lysate.
Description de la cible
~50 kDa observed. 47.33/50.20 kDa (human mature/pro-nucleobindin-2), 47.42/50.30 kDa (mouse mature/pro-nucleobindin-2), 47.25/50.09 kDa (rat mature/pro-nucleobindin-2), 9.552/9.612/9.583 kDa (human/mouse/rat nesfatin-1) calculated.
Forme physique
Format: Purified
Protein G purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stockage et stabilité
Stable for 1 year at 2-8°C from date of receipt.
Autres remarques
Concentration: Please refer to lot specific datasheet.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Aristea Psilopanagioti et al.
Journal of neuroendocrinology, 32(9), e12899-e12899 (2020-09-10)
Feeding is a complex behaviour entailing elaborate interactions between forebrain, hypothalamic and brainstem neuronal circuits via multiple orexigenic and anorexigenic neuropeptides. Nucleobindin-2 (NUCB2)/nesfatin-1 is a negative regulator of food intake and body weight with a widespread distribution in rodent brainstem
Aristea Psilopanagioti et al.
International journal of molecular sciences, 23(23) (2022-12-12)
Data on animals emphasize the importance of the neuronal glucagon-like peptide-1 (GLP-1) receptor (GLP-1R) for feeding suppression, although it is unclear whether astrocytes participate in the transduction of anorectic GLP-1R-dependent signals. In humans, the brain circuitry underlying these effects remains
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