MABN704
Anti-ASS1 Antibody, clone 2C10
clone 2C10, from mouse
Synonyme(s) :
Argininosuccinate synthase, Citrulline--aspartate ligase, ASS1
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
2C10, monoclonal
Espèces réactives
mouse, human, rat, monkey
Technique(s)
immunohistochemistry: suitable
western blot: suitable
Isotype
IgG1
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... ASS1(445)
Description générale
Argininosuccinate synthase (ASS1) or alternatively known by the older name of Citrulline-Aspartate ligase is encoded by the ASS1/ASS gene in humans and is the enzyme that is responsible for the third step in the urea cycle critical to the metabolism of nitrogen. ASS1 catalyzes the synthesis of argininosuccinate from citrulline and aspartate. Argininosuccinate is the immediate precursor of arginine and ASS1 was first identified as the rate limiting step in the liver for this reaction. ASS1 is now recognized to be expressed ubiquitously in mammalian tissues. ASS1 also performs the rate limiting step for NO production in the citrulline-NO cycle as well. Regulation of ASS1 expression can vary depending upon the tissue examined. In the liver, where arginine is hydrolyzed to form urea and ornithine, ASS1 is highly expressed, and various hormones such as glucocorticoids, glucagon, insulin and even high dietary levels of specific amino acids like glutamine regulate expression of ASS1 during development and adult life. In other tissues and in particular in nitric oxide (NO) producing cells, ASS1 is expressed at a low level but induced by proinflammatory cytokines. Interestingly ASS1 expression is also increased in Alzheimer’s disease in both neurons and glia, which also is correlated with iNOS expression and suggests a link between increased NO production and iNOS expression observed in Alzheimer brains. EMD-Millipore’s Anti-ASS1 monoclonal antibody has been tested in western blot on recombinant protein and cell lysates from A431, Raji, MOLT4, Jurkat, A549, NIH3T3, PC-12, and COS7 and in paraffin embedded immunohistochemistry on cervical cancer tissue sections. The antibody has also been tested in direct ELISA on recombinant antigen.
Immunogène
Purified recombinant fragment of human ASS1 expressed in E. Coli.
Application
Detect Argininosuccinate synthase using this mouse monoclonal antibody, Anti-ASS1 Antibody, clone 2C10 validated for use in western blotting & IHC.
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected ASS1 in human cervical cancer and colon cancer tissues.
Optimal working dilutions must be determined by end user.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neurodegenerative Diseases
Qualité
Evaluated by Western Blotting in A431, Raji, MOLT4, Jurkat, A549, NIH/3T3, PC-12 and Cos7 cell lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected ASS1 in A431, Raji, MOLT4, Jurkat, A549, NIH/3T3, PC-12 and Cos7 cell lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected ASS1 in A431, Raji, MOLT4, Jurkat, A549, NIH/3T3, PC-12 and Cos7 cell lysates.
Description de la cible
~47 kDa observed. Uncharacterized bands may appear in some lysate(s).
Forme physique
Format: Purified
Protein G Purified
Purified mouse monoclonal in PBS with up to 0.1% sodium azide and 0.5% protein stabilizer.
Stockage et stabilité
Stable for 1 year at 2-8°C from date of receipt.
Remarque sur l'analyse
Control
A431, Raji, MOLT4, Jurkat, A549, NIH/3T3, PC-12 and Cos7 cell lysates
A431, Raji, MOLT4, Jurkat, A549, NIH/3T3, PC-12 and Cos7 cell lysates
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Sarah E Barnett et al.
Molecular cancer research : MCR, 21(5), 411-427 (2023-01-21)
The nuclear deubiquitylase BRCA1-associated protein 1 (BAP1) is frequently inactivated in malignant pleural mesothelioma (MPM) and germline BAP1 mutation predisposes to cancers including MPM. To explore the influence on cell physiology and drug sensitivity, we sequentially edited a predisposition mutation
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