MABF920

Anti-SAP/SH2D1A Antibody, clone 1A9

clone 1A9, from rat

• Synonyme(s) : SH2 domain-containing protein 1A, Signaling lymphocytic activation molecule-associated protein, SLAM-associated protein, T-cell signal transduction molecule SAP
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rat
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 1A9, monoclonal
• Espèces réactives: mouse, human
• Technique(s): flow cytometry: suitable; western blot: suitable
• Isotype: IgG2aκ
• Numéro d'accès NCBI: NP_035494
• Numéro d'accès UniProt: O88890
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... SH2D1A(4068)

Description

Description générale

SH2 domain-containing protein 1A (UniProt O60880; also known as Signaling lymphocytic activation molecule-associated protein, SLAM-associated protein, T-cell signal transduction molecule SAP) is encoded by the Sh2d1a (also known as Xlp) gene (Gene ID 20400) in murine species. SAP/SH2D1A is a 628-amino acid (isoform A) adaptor protein expressed in T cells, natural killer (NK) cells, NK-T cells, and some B cells. SAP/SH2D1A is made up almost exclusively of a Src homology 2 (SH2) domain (a.a. 6-104) and plays an important role in multiple immune cell functions, including T helper 2 (TH2) cytokine production, T cell-dependent antibody production, follicular T helper (TFH) cell differentiation, NK-T cell development, CD8+ T cell- and NK cell-mediated cytotoxicity. SAP couples signaling lymphocytic activation molecule (SLAM) family receptors to tyrosine phosphorylation signals through its interaction with the SH3 domain of Src family protein tyrosine kinase Fyn. In addition, SAP can prevent SH2 domain-containing negative regulators, such as phosphatases SHIP-1 and SHP-1, from inactivating SLAM-related receptors by blocking their interaction with the receptors. Evidence also shows that SAP/SH2D1A regulates T cell-dependent antibody production and TFH cell differentiation independent of Fyn. SAP/SH2D1A gene is mutated and inactivated in X-linked lymphoproliferative disease, a rare human immunodeficiency characterized by an uncontrolled immune response to Epstein-Barr virus infection.

Spécificité

Clone 1A9 reacts with SAP, but not EAT-2 or ERT (Roncagalli, R., et al. (2005). Nat. Immunol. 6(10):1002-1010). Clone 1A9 immunostained wild-type, but not sh2d1a-knockout murine peripheral blood CD3+CD4+ lymphocytes (Zhong, M.C., and Veillette, A. (2013). Mol. Cell. Biol. 33(6):1223-1232).

Immunogène

GST-tagged recombinant full-length mouse SAP.

Application

Anti-SAP Antibody, clone 1A9 is an antibody against SH2D1A for use in Flow Cytometry, Western Blotting.

Research Category
Inflammation & Immunology

Research Sub Category
Immunological Signaling

Western Blotting Analysis: A representative lot detected SAP in YT-S human NK cell lysates (Pérez-Quintero, L.A., et al. (2014). J. Exp. Med. 211(4):727-742).
Flow Cytometry Analysis: A representative lot immunostained wild-type, but not sh2d1a-knockout, murine peripheral blood CD3+CD4+ lymphocytes (Zhong, M.C., and Veillette, A. (2013). Mol. Cell. Biol. 33(6):1223-1232).
Western Blotting Analysis: A representative lot detected SAP, but not EAT-2 or ERT, exogenously expressed in the mouse mature mouse T cell line BI-141 (Roncagalli, R., et al. (2005). Nat. Immunol. 6(10):1002-1010).

Qualité

Evaluated by Flow Cytometry in mouse splenocytes.

Flow Cytometry Analysis: 0.1 µg of this antibody detected SAP/SH2D1A in mouse splenocytes.

Description de la cible

13.90/13.59 kDa (murine isoform 1/2) and 14.19 kDa (human isoform A) calculated.

Forme physique

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable