MABF3036

Anti-PSGL-1/CD162 Antibody, clone HECA-452

• Synonyme(s) : P-selectin glycoprotein ligand 1;PSGL-1 Selectin P ligand,;CD162
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: rat
• Niveau de qualité: 200
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: HECA-452, monoclonal
• Poids mol.: calculated mol wt 43.2 kDa
• Produit purifié par: affinity chromatography
• Espèces réactives: human
• Conditionnement: antibody small pack of 100 μg
• Technique(s): flow cytometry: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotype: IgMκ
• Séquence de l'épitope: Extracellular domain
• Numéro d'accès Protein ID: NP_001193538
• Numéro d'accès UniProt: Q14242
• Température de stockage: -10 to -25°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... SELPLG(6404)

Description

Description générale

P-selectin glycoprotein ligand 1 (UniProt: Q14242; also known as PSGL-1 Selectin P ligand, CD162) is encoded by the SELPLG gene (Gene ID: 6404) in human. Leukocytes express L-selectin ligands that are critical for leukocyte-leukocyte interactions at sites of inflammation. PSGL-1 is a predominant leukocyte L-selectin ligand that displays appropriate sialyl Lewis x (sLex)-like carbohydrate determinants for L-selectin recognition. It is a single-pass, mucin-like type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-17), which is subsequently cleaved off to produce a form that contains an extracellular domain (18-320), a transmembrane domain (321-341), and a cytoplasmic domain (342-412). The mature form is generated following the cleaved of the propeptide (aa 18-41) region. PSGL-1 is a SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. It is constitutively expressed on all human peripheral-blood T cells and is also expressed on skin-homing T cells. Clone HECA-452 can detect a human endothelial cell differentiation antigen (HECA) selectively expressed on high endothelium. In addition to reacting with high endothelium, it also shows some cross-reactivity with a set of monocytic cells. This clone is shown to block the majority of P-selectin binding to neutrophils. (Ref.: Tu, L., et al. (1999). J. Immunol. 163(9); 5070-5078; Fuhlbrigge, RC., et al. (1997). Nature. 389(6654); 978-981; Duivestijn, AM., et al. (1988). Am. J. Pathol. 130(1); 147-155).

Spécificité

Clone HECA-452 is a rat monoclonal antibody that detects PSGL-1/CD162. It targets an epitope within the extracellular domain.

Immunogène

Stromal preparation from human tonsil tissue.

Application

Quality Control Testing

Evaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC).

Flow Cytometry Analysis (FC): 1.0 μg of this antibody stained one million Human peripheral blood mononuclear cells (PBMC).

Tested Applications

Immunohistochemistry Applications: A representative lot detected PSGL-1/CD162 in Immunohistochemistry application (Duijvestijn, A.M., et al. (1988). Am J Pathol. 130(1):147-55).

Flow Cytometry Analysis: A representative lot detected PSGL-1/CD162 in Flow Cytometry application (Picker, L.J., et al. (1991). Nature. 349(6312):796-9; Fuhlbrigge, R.C., et al. (1997). Nature. 389(6654):978-81).

Western Blotting Analysis: A representative lot detected PSGL-1/CD162 in Western Blotting application (Fuhlbrigge, R.C., et al. (1997). Nature. 389(6654):978-81).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Forme physique

Purified rat monoclonal antibody IgM in PBS without azide.

Reconstitution

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable