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MAB8560

Sigma-Aldrich

Anti-Poliovirus 1 Antibody, clone 583-G8-G2-A4

ascites fluid, clone 583-G8-G2-A4, Chemicon®

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

300

Forme d'anticorps

ascites fluid

Type de produit anticorps

primary antibodies

Clone

583-G8-G2-A4, monoclonal

Espèces réactives

human

Fabricant/nom de marque

Chemicon®

Technique(s)

immunofluorescence: suitable

Isotype

IgG1

Conditions d'expédition

wet ice

Spécificité

Reacts with the Poliovirus 1.

Application

Detect Poliovirus 1 using this Anti-Poliovirus 1 Antibody, clone 583-G8-G2-A4 validated for use in IF.
IFA at 1:100-1:400.

Optimal working dilutions must be determined by end user.

SUGGESTED IFA

PROTOCOL:

Substrate slides:

1. Scrape and spot cells onto microscope slide wells.

2. Air Dry.

3. Fix in cold acetone for 10 minutes at +4°C.

Stain:

1. Add 15 μL diluted polio antibody.

2. Incubate at +37°C for 30 minutes in a humid chamber.

3. Rinse in PBS for 5 minutes; repeat with fresh PBS.

4. Add 15 μL diluted labeled antibody.

5. Repeat steps 2 and 3

6. Mount with buffered glycerol.
Research Category
Infectious Diseases
Research Sub Category
Infectious Diseases - Viral

Stockage et stabilité

Store at -20°C for up to 12 months in convenient aliquots. Avoid repeated freeze/thaw cycles.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

William C Weldon et al.
Methods in molecular biology (Clifton, N.J.), 1387, 145-176 (2016-03-18)
Testing for neutralizing antibodies against polioviruses has been an established gold standard for assessing individual protection from disease, population immunity, vaccine efficacy studies, and other vaccine clinical trials. Detecting poliovirus specific IgM and IgA in sera and mucosal specimens has
Mohsan Saeed et al.
PLoS pathogens, 16(9), e1008927-e1008927 (2020-10-01)
Viruses cleave cellular proteins to remodel the host proteome. The study of these cleavages has revealed mechanisms of immune evasion, resource exploitation, and pathogenesis. However, the full extent of virus-induced proteolysis in infected cells is unknown, mainly because until recently

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