MAB1393-I

Anti-PECAM-1 (CD31) Antibody, clone TLD-3A12

clone TLD-3A12, from mouse

• Synonyme(s) : Platelet endothelial cell adhesion molecule, CD31
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: TLD-3A12, monoclonal
• Espèces réactives: human, rat
• Technique(s): ELISA: suitable; flow cytometry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_113779
• Numéro d'accès UniProt: Q3SWT0
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... PECAM1(5175) ; rat ... Pecam1(29583)

Description

Description générale

Platelet endothelial cell adhesion molecule (UniProt: Q3SWT0; PECAM-1, CD31) is encoded by the Pecam1 (also known as Pecam) gene (Gene ID: 29583) in rat.PECAM-1 is a single-pass type I membrane protein that contains 6 Ig-like C2-type (immunoglobulin-like) domains. PECAM1 is differentially glycosylated involving both N-linked and O-linked glycosylation sites. It is a cell adhesion molecule that is required for leukocyte trans-endothelial migration (TEM) under most inflammatory conditions. PECAM1 plays a vital role in signaling processes involved in angiogenesis, platelet function, thrombosis, mechanosensing of endothelial cell response to fluid shear stress, and regulation of multiple stages of leukocyte migration through venular walls. PECAM1 is expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. PECAM1 prevents phagocyte ingestion of closely apposed viable cells by transmitting ′detachment′ signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes. During apoptosis, the inside-out signaling of PECAM1 is disabled that allows the apoptotic cell to accept phagocyte interaction. PECAM1 is phosphorylated on serine and tyrosine residues following cellular activation. In endothelial cells Fyn is shown to mediate mechanical-force induced tyrosine phosphorylation. In response to Fc epsilon RI (FCER1 activation), PECAM1 is phosphorylated on tyrosine residues by FER and FES.

Spécificité

Clone TLD-3A12 recognizes rat and human PECAM-1.

Immunogène

Lewis rat derived activated microglial cells.

Application

Immunoprecipitation Analysis: A representative lot detected PECAM-1 (CD31) in Immunoprecipitation applications (Williams, K.C., et. al. (1996). J Neurosci Res. 45(6):747-57).

Flow Cytometry Analysis: 1 µg from a representative lot detected PECAM-1 (CD31) in one million rat splenocytes.

Western Blotting Analysis: A representative lot detected PECAM-1 (CD31) in Western Blotting applications (Male, D., et. al. (1995). Immunology. 84(3):453-60).

ELISA Analysis: A representative lot detected PECAM-1 (CD31) in ELISA applications (Male, D., et. al. (1995). Immunology. 84(3):453-60; Williams, K.C., et. al. (1996). J Neurosci Res. 45(6):747-57).

Affects Function: A representative lot of PECAM-1 (CD31) Affected Function (Williams, K.C., et. al. (1996). J Neurosci Res. 45(6):747-57).

Immunohistochemistry Analysis: A representative lot detected PECAM-1 (CD31) in Immunohistochemistry applications (Williams, K.C., et. al. (1996). J Neurosci Res. 45(6):747-57).

Research Category
Cell Structure

This mouse monoclonal Anti-PECAM-1 (CD31), clone TLD-3A12, Cat. No. MAB1393-I is tested for use in ELISA, Flow Cytometry, Functional Studies, Immunohistochemistry (Paraffin) and Immunohistochemistry, Immunoprecipitation, and Western Blotting, for the detection of CD31/PECAM-1.

Qualité

Evaluated by Immunohistochemistry in human tonsil.

Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected PECAM-1 (CD31) in human tonsil tissue.

Description de la cible

76.19 kDa calculated.

Forme physique

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in PBS without azide.

Stockage et stabilité

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable