AP501B

Goat Anti-Mouse IgM+IgG+IgA (H+L) Antibody, biotin conjugate, Species Adsorbed

Chemicon^®, from goat

• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.46

Propriétés

• Source biologique: goat
• Niveau de qualité: 100
• Conjugué: biotin conjugate
• Forme d'anticorps: affinity purified immunoglobulin
• Type de produit anticorps: secondary antibodies
• Clone: polyclonal
• Espèces réactives: mouse
• Fabricant/nom de marque: Chemicon^®
• Technique(s): ELISA: suitable; immunofluorescence: suitable
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified

Description

Description générale

IgG is the most abundant immunoglobulin compose of two heavy chains and two light chains. Each molecule has two antigen binding sites. IgG has 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). IgM constitutes about 10% of serum immunoglobulins. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces.

Spécificité

Reacts with heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA. Absorbed for human sera and purified human paraproteins. Minimal cross reactivity with human immunoglobulins. Reactivity with other species has not been determined.

The antibody reacts with the heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with human immunoglobulins.

Immunogène

Epitope: Mouse IgM, IgG, IgA

Prepared from pooled antisera from goats hyperimmunized with mouse IgM, IgG and IgA paraproteins.

Application

Detect Mouse IgM+IgG+IgA (H+L) using this Goat anti-Mouse IgM+IgG+IgA (H+L) Antibody, biotin conjugate, Species Adsorbed validated for use in ELISA & IF.

Immunofluorescence: ≤1 μg/10E6 cells
ELISA: 1:5,000-1:20,000

Optimal working dilutions must be determined by the end user.

Research Category
Secondary & Control Antibodies

Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling

Forme physique

Affinity Chromatography

Purified by affinity chromatography on pooled mouse IgM+IgG+IgA covalently linked to agarose. Liquid in 2.0 mL PBS/NaN3.

Stockage et stabilité

Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable