ABT1838

Anti-Claudin-16

from rabbit, purified by affinity chromatography

• Synonyme(s) : Paracellin-1, PCLN-1
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: canine, human
• Technique(s): immunocytochemistry: suitable; immunofluorescence: suitable
• Isotype: IgG
• Numéro d'accès NCBI: NP_006571
• Numéro d'accès UniProt: Q9Y5I7
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... CLDN16(10686)

Description

Description générale

Claudin-16 (UniProt: Q9Y5I7; also known as Paracellin-1, PCLN-1) is encoded by the CDLN1 (also known as PCLN1) gene (Gene ID: 10686) in human. Claudin-16 is a multi-pass membrane protein that span the plasma membrane 4 times, with their N- and C-termini located in the cytosol. It is selectively expressed at tight junctions of renal epithelial cells of the thick ascending limb of loop of Henle s where it acts as either an intercellular pore or ion concentration sensor to regulate the paracellular resorption of divalent cations. Claudin-16 may form, alone or in partnership with other constituents, an intercellular pore permitting paracellular passage of magnesium and calcium ions down their electrochemical gradients. Alternatively, it could be a sensor of magnesium concentration that could alter paracellular permeability mediated by other factors. Over 20 different mutation sin CLDN1 gene have been reported that are linked to familial hypomagnesemia with hypercalciuria and nephrocalcinosis (FHHNC), a renal Mg2+ and Ca2+ wasting disorder that ultimately leads to progressive kidney failure. (Ref.: Muller D et al. (2006). J. Clin. Endocrinol. Metab. 91(8):3076-3079).

Spécificité

This rabbit polyclonal antibody detects Claudin-16 in human and canine cells. It targets an epitope within 15 amino acids from the N-terminal region.

Immunogène

Conjugated linear peptide corresponding to 15 amino acids from the N-terminal region of human Claudin-16.

Epitope: extracellular domain

Application

Immunofluorescence Analysis: A representative lot detected Claudin-16 in Immunofluorescence applications (Kausalya, P.J., et. al. (2006). J Clin Invest. 116(4):878-91).

Inhibits Activity/Function: A representative lot blocked Clathrin-mediated endocytosis in MDCK cells (Muller, D., et. al. (2006). J Clin Endocrinol Metab. 91(8):3076-9).

Research Category
Cell Structure

This rabbit polyclonal Anti-Claudin-16, Cat. No. ABT1838, is a rabbit polyclonal antibody that detects Claudin-16 and has been tested for use in Immunocytochemistry, Immunofluorescence, and Inhibition/Function studies.

Qualité

Evaluated by Immunocytochemistry in MDCK cells.

Immunocytochemistry Analysis: A 1:25 dilution of this antibody detected Claudin-16 in Madin-Darby Canine Kidney Epithelial (MDCK) cells.

Description de la cible

33.84 kDa calculated.

Forme physique

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.54 M Tris, 0.2 M Glycine, 250 mM NaCl, pH 7.5 without azide.

Stockage et stabilité

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): does not flash
• Point d'éclair (°C): does not flash