ABN912

Anti-Myelin Basic Protein

from rabbit, purified by affinity chromatography

• Synonyme(s) : MBP
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: rabbit
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human, rat
• Réactivité de l'espèce (prédite par homologie): guinea pig (based on 100% sequence homology)
• Conditionnement: antibody small pack of 25 μg
• Technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• Numéro d'accès UniProt: P25188
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... MBP(4155) ; rat ... Mbp(24547)

Description

Description générale

Myelin basic protein (UniProt: also known as MBP) is encoded by the MBP gene in guinea pig. MBP is a homodimeric protein that is found in both the central and the peripheral nervous system. It is one of the most abundant protein component of the myelin membrane in the CNS and plays a role in both the formation and stabilization of this myelin membrane. It is responsible for adhesion of the cytosolic surfaces of multilayered compact myelin. At least 5 charge isomers (C1, C2, C3, C4, and C5) are reported to be produced because of optional post-translational modifications such as phosphorylation of serine or threonine residues, deamidation of glutamine or asparagine residues, citrullination and methylation of arginine residues. Of these C1 isomer is the most cationic, least modified, and most abundant form and C5 is the least cationic form. C1 and C2 are reported to be unphosphorylated, C3 and C4 are monophosphorylated, and C5 is phosphorylated at two positions. Phosphorylation is achieved with the help of TAOK2, VRK2, MAPK11, MAPK12, MAPK14, and MINK1). MBP can interact with many polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and with negatively charged lipids. (Ref.: Boggs, JM (2006). Cell. Mol. Life Sci. 63(17); 1945-61).

Spécificité

This rabbit polyclonal antibody detects myelin basic protein. It targets an epitope within 19 amino acids from the internal region.

Immunogène

KLH-conjugated linear peptide corresponding to 19 amino acids from the internal region of Guinea pig myelin basic protein.

Application

Anti-Myelin Basic Protein Antibody, Cat. No. ABN912, is a highly specific rabbit polyclonal antibody that targets Myelin basic protein and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Myelin Basic Protein in human cerebral cortex and rat cerebellum tissue sections.

Research Category
Neuroscience

Qualité

Evaluated by Western Blotting in human cerebellum tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Myelin Basic Protein in human cerebellum tissue lysate.

Description de la cible

~17 kDa observed; 18.21 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.