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ABE1338

Sigma-Aldrich

Anti-METTL14 Antibody

from rabbit, purified by affinity chromatography

Synonyme(s) :

N6-adenosine-methyltransferase subunit METTL14, m6A methyltransferase METTL14, Methyltransferase-like protein 14

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

100

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Produit purifié par

affinity chromatography

Espèces réactives

mouse, human

Réactivité de l'espèce (prédite par homologie)

zebrafish (based on 100% sequence homology), rat (based on 100% sequence homology)

Technique(s)

western blot: suitable

Numéro d'accès NCBI

NP_066012

Numéro d'accès UniProt

Q9HCE5

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... METTL14(57721)

Description générale

N6-adenosine-methyltransferase subunit METTL14 (EC 2.1.1.62; UniProt Q9HCE5; also known as m6A methyltransferase METTL14, Methyltransferase-like protein 14) is encoded by the METTL14 (also known as KIAA1627) gene (Gene ID 57721) in human. N(6)-Methyladenosine (m(6)A) represents the most prevalent internal modification in messenger and long noncoding RNAs. This reversible modification is installed by the m(6)A methylation "writers" and remomved by demethylases that serve as "erasers. In addition, methyl-specific binding proteins, primarily of the YTH-domain family, function as "readers" and mediate the effect by binding to modified transcripts. METTL14 is a component of the nuclear methyltransferase complex (writers) composed of METTL3, METTL14, and WTAP. At least 2 demethylases (erasers), ALKBH5 and FTO, that can catalyze the reverse reaction. The roles of m(6)A in cell fates regulation and embryonic development highlight the existence of another layer of epigenetic regulation at the RNA level.

Spécificité

The immunogen sequence is present in human METTL14 (UniProt Q9HCE5) and both of murine METTL14 spliced isoforms (Uniprot Q3UIK4).

Immunogène

KLH-conjugated linear peptide corresponding to an internal sequence from the C-terminal half of human METTL14.

Application

This Anti-METTL14 Antibody is validated for use in Western Blotting for the detection of METTL14.

Qualité

Evaluated by Western Blotting in Raji nuclear extract.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected METTL14 in 10 µg of Raji nuclear extract.

Description de la cible

~56 kDa observed. 52.15 kDa (human), 52.12/40.72 kDa (mouse isoform 1/2) calculated. Uncharacterized band(s) may appear in some lysates.

Autres remarques

Concentration: Please refer to lot specific datasheet.

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Réf. du produit
Description
Tarif

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Loss of METTL3 attenuates blastic plasmacytoid dendritic cell neoplasm response to PRMT5 inhibition via IFN signaling.
Rethnam, et al.
Blood advances, 6, 5330-5344 (2023)
Fanpeng Zhao et al.
Molecular neurodegeneration, 16(1), 70-70 (2021-10-02)
N6-methyladenosine (m6A) modification of RNA influences fundamental aspects of RNA metabolism and m6A dysregulation is implicated in various human diseases. In this study, we explored the potential role of RNA m6A modification in the pathogenesis of Alzheimer disease (AD). We

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