ABC157

Anti-MCM7 Antibody

from rabbit

• Synonyme(s) : DNA replication licensing factor MCM7, CDC47 homolog, P1.1-MCM3
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Espèces réactives: human, rat
• Réactivité de l'espèce (prédite par homologie): rhesus macaque (based on 100% sequence homology), chimpanzee (based on 100% sequence homology)
• Technique(s): western blot: suitable
• Numéro d'accès NCBI: NP_005907
• Numéro d'accès UniProt: P33993
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... MCM7(4176)

Description

Description générale

MCM7 is a highly conserved mini-chromosome maintenance protein (MCM) that is involved in the initiation of eukaryotic genome replication. It forms a hexameric complex with MCM4 and MCM6 (two subunits of MCM7, two subunits of MCM4 and two subunits of MCM7) and this complex regulates the helicase activity of the pre-replication complex. This complex is also involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. MCM7 is reported to bind to DNA in metaphase II in oocytes and remains associated with the DNA until the S-phase. Two isoforms of MCM7 have been reported, 61 kDa and 90 kDa. In neuroblastoma tumors MCM7 expression is shown to correlates with MYCN (v-myc myelocytomatosis viral related oncogene, neuroblastoma derived) amplification where in MCM7 is rapidly up-regulated by Inducible MYCN Expression. MYCN protein is shown to bind specifically to the MCM7 promoter region, which possesses an E-box binding site for the Myc oncoproteins.

Spécificité

Other homologies: Mouse (71% sequence homology).

This antibody recognizes the N-terminus of MCM7

Immunogène

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to the N-terminus of human MCM7.

Application

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This MCM7 antibody is validated for use in WB for the detection of the MCM7 protein.

Western Blot Analysis: 0.5 µg/mL from a representative lot detected MCM7 in 10 µg of L6 cell lysate.

Qualité

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected MCM7 in 10 µg of HeLa cell lysate.

Description de la cible

~82 kDa observed

Liaison

Replaces: MABE188

Forme physique

Format: Purified

Protein A purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
HeLa cell lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable