AB5246
Anti-P2X7 Receptor Antibody
Chemicon®, from rabbit
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Clone:
polyclonal
application:
IHC
WB
WB
Espèces réactives:
rat
Technique(s):
immunohistochemistry: suitable
western blot: suitable
western blot: suitable
citations:
9
Produits recommandés
Source biologique
rabbit
Forme d'anticorps
affinity purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
polyclonal
Produit purifié par
affinity chromatography
Espèces réactives
rat
Fabricant/nom de marque
Chemicon®
Technique(s)
immunohistochemistry: suitable
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... P2RX7(5027)
Spécificité
The epitope is specific for P2X7 and is not present in any other known protein.
SPECIES REACTIVITIES: Rat, mouse (Solee et al. 2001) and Candida albicans (Koshlukova et al. 2000). The sequence is highly conserved in mouse and human (18/20 and 16/20 residues identical, respectively). Reactivity with other species has not been tested.
SPECIES REACTIVITIES: Rat, mouse (Solee et al. 2001) and Candida albicans (Koshlukova et al. 2000). The sequence is highly conserved in mouse and human (18/20 and 16/20 residues identical, respectively). Reactivity with other species has not been tested.
Immunogène
Purified peptide P2X7 from 576-595 of rat P2X7 (Koshlukova et al. 2000).
Application
Anti-P2X7 Receptor Antibody detects level of P2X7 Receptor & has been published & validated for use in IH & WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neurotransmitters & Receptors
Western blot:1:200-1:1000 using ECL on rat brain membranes.
Immunohistochemistry (Vial & Evans 2000; Lewis & Evans 2000; Gitterman & Evans 2000)
Dilutions should be made using a carrier protein such as BSA (1-3%)
Optimal working dilutions must be determined by the end user.
Immunohistochemistry (Vial & Evans 2000; Lewis & Evans 2000; Gitterman & Evans 2000)
Dilutions should be made using a carrier protein such as BSA (1-3%)
Optimal working dilutions must be determined by the end user.
Forme physique
Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
Stockage et stabilité
Maintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Remarque sur l'analyse
Control
CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile distilled water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile distilled water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Mentions de danger
Conseils de prudence
Classification des risques
Aquatic Chronic 3
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Jonathan R Genzen et al.
Purinergic signalling, 5(3), 299-307 (2009-03-11)
Ependymal cells line the cerebral ventricles and are located in an ideal position to detect central nervous system injury and inflammation. The signaling mechanisms of ependymal cells, however, are poorly understood. As extracellular adenosine 5'-triphosphate is elevated in the context
Paola Chiozzi et al.
Scientific reports, 9(1), 6475-6475 (2019-04-26)
Previous data from our laboratory show that expression of the P2X7 receptor (P2X7R) is needed for amyloid β (Aβ)-stimulated microglia activation and IL-1β release in vitro and in vivo. We also showed that Aβ-dependent stimulation is inhibited by the dihydropyridine
Anna Lisa Giuliani et al.
Frontiers in immunology, 10, 793-793 (2019-04-30)
The P2X7 receptor (P2X7R) is a key pro-inflammatory plasma membrane receptor responsible for NLRP3 inflammasome activation and IL-1β release. Various inflammatory plasma membrane receptors (e.g., IL-1 type I receptor, TNF type I and II receptors, IL-2 receptor) are shed under
Maria Pina Notarangelo et al.
Frontiers in cell and developmental biology, 12, 1368318-1368318 (2024-04-19)
A comprehensive understanding of the molecules that play key roles in the physiological and pathological homeostasis of the human intervertebral disc (IVD) remains challenging, as does the development of new therapeutic treatments. We recently found a positive correlation between IVD
Ana A Q A Santos et al.
World journal of gastroenterology, 28(30), 4075-4088 (2022-09-27)
Clostridioides difficile (C. difficile) is the most common pathogen causing health care-associated infections. C. difficile TcdA and TcdB have been shown to activate enteric neurons; however, what population of these cells is more profoundly influenced and the mechanism underlying these
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