AB3594
Anti-Aquaporin 4 Antibody, CT
Chemicon®, from rabbit
Synonyme(s) :
Mercurial-Insensitive Water Channel
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Espèces réactives
mouse, human, rat
Fabricant/nom de marque
Chemicon®
Technique(s)
immunohistochemistry: suitable
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... AQP4(361)
Description générale
Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special proteins "aquaporins". Aquaporins, are integral membrane proteins from a larger family of major intrinsic proteins (MIP) that form pores in the membrane of biological cells. Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. The water-specific channel Aquaporin 4 is located in various tissues including the gastrointestinal tract, kidney and brain. It is an osmoreceptor which regulates body water balance and mediates water flow within the central nervous system. In CNS tissue, AQP4 localizes to astrocyte endfeet making it a useful structural marker.
Spécificité
Reactivity with other species has not yet been tested. It is expected that the antibody may also react with bovine (71/75) and rabbit (64/75) due to sequence homology.
Recognizes Aquaporin 4 (AQP4, WCH4, Mercurial-Insensitive Water Channel).
Immunogène
Epitope: C-terminus
GST fusion protein with residues 249-323 of rat Aquaporin 4 (SwissProt accession number P47863).
Application
Detect Aquaporin 4 using this Anti-Aquaporin 4 Antibody, C-terminus validated for use in IH & WB.
Immunohistochemistry:
A previous lot of this antibody was used on rat brain sections.
Dilutions should be made using a carrier protein such as BSA (1-3%).
Western blotting:
1:500-1:1,000 using ECL on rat brain membranes.
Optimal working dilutions must be determined by the end user
A previous lot of this antibody was used on rat brain sections.
Dilutions should be made using a carrier protein such as BSA (1-3%).
Western blotting:
1:500-1:1,000 using ECL on rat brain membranes.
Optimal working dilutions must be determined by the end user
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion & Transport Channels
Ion & Transport Channels
Qualité
Routinely evaluated by Western Blot on Mouse Brain lysates.
Western Blot Analysis: 1:500 dilution of this lot detected AQUAPORIN 4 on 10 μg of Mouse Brain lysates.
Western Blot Analysis: 1:500 dilution of this lot detected AQUAPORIN 4 on 10 μg of Mouse Brain lysates.
Description de la cible
38 kDa
Forme physique
Format: Purified
ImmunoAffinity Purified: Amber vial of Rb X Aquaporin 4, C-Terminus (part # AB3594-50ULa)
Purified rabbit polyclonal, lyophilized from buffer containing PBS, pH 7.4, containing 1% BSA, and 0.05% sodium azide. Reconstitute with 200 µL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).
Stockage et stabilité
Stable for up to 6 months at -20ºC from date of receipt. After reconstitution stable at -20ºC in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Remarque sur l'analyse
Control
Clear vial of RbX Aquaporin 4, CT Control Antigen(AB3594-50ULb) included free of charge with the antibody is 120 µg of control antigen (lyophilized powder). Reconstitute with 100 µL of PBS. For positive control, in Western blot use 20 ng of protein per Minigel lane. For negative control, preincubate 3 µg of purified peptide with 1 µg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Clear vial of RbX Aquaporin 4, CT Control Antigen(AB3594-50ULb) included free of charge with the antibody is 120 µg of control antigen (lyophilized powder). Reconstitute with 100 µL of PBS. For positive control, in Western blot use 20 ng of protein per Minigel lane. For negative control, preincubate 3 µg of purified peptide with 1 µg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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