AB3046
Anti-Uncoupling Protein 3 Antibody
Chemicon®, from rabbit
Synonyme(s) :
UCP3
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
affinity purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
polyclonal
Produit purifié par
affinity chromatography
Espèces réactives
human, rat
Fabricant/nom de marque
Chemicon®
Technique(s)
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... UCP3(7352)
rat ... Ucp3(25708)
Description générale
Human UCP3 long form (UCP3L) is a 312 aa mitochondrial uncoupling protein. It is only 57% and 73% homologous with human UCP1 and UCP2 respectively. Like other UCPs, UCP3 is predicted to contain 6 transmembrane domains. The UCP3S lacks the 6th transmembrane domain and is only 275 aa long. UCP3 has preferential expression in muscle and it is unaffected by cold acclimation.
Spécificité
Uncoupling Protein 3 (UCP3), human. It is expected that the antibody will recognize both the long and short forms (30-33 kDa & 24-27 kDa) however splice variants are expected and precise reactivity profile has yet to be determined. Human UCP3 long form (UCP3L) is a 312 aa mitochondrial uncoupling protein (Boss et al. 1997; Vidal-Puig et al. 1997). It is only 57% and 73% homologous with human UCP1 and UCP2 respectively. Like other UCPs, UCP3 is predicted to contain 6 transmembrane domains. The UCP3S lacks the 6th transmembrane domain and is only 275 aa long (Vidal-Puig et al. 1997). UCP3 has preferential expression in muscle and it is unaffected by cold acclimation.
Immunogène
14 amino acid peptide sequence (designated UCP32) mapping near the C-terminus of human UCP3 (Boss et al. 1997). The UCP32 peptide has no significant homology with UCP1 or UCP2.
Application
Anti-Uncoupling Protein 3 Antibody is an antibody against Uncoupling Protein 3 for use in ELISA, IH & WB.
Research Category
Metabolism
Metabolism
Research Sub Category
Ion & Transport Channels
Ion & Transport Channels
Western Blotting: 1-10 μg/mL using Chemiluminescence technique.
Immunohistochemistry: 2-20 μg/mL on formaldehyde fixed tissue.
ELISA: (1:10,000-1:100,000 using 50-100 ng UCP32 control peptide/well).
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 2-20 μg/mL on formaldehyde fixed tissue.
ELISA: (1:10,000-1:100,000 using 50-100 ng UCP32 control peptide/well).
Optimal working dilutions must be determined by end user.
Description de la cible
30-33 kDa & 24-27 kDa
Forme physique
Affinity Purified immunoglobulin. Liquid in PBS with 0.1% BSA.
ImmunoAffinity Purified
Stockage et stabilité
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Remarque sur l'analyse
Control
Muscle tissue, Blocking peptide for UCP3 is available (Catalogue No. AG769).
Muscle tissue, Blocking peptide for UCP3 is available (Catalogue No. AG769).
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Helena Gustafsson et al.
Journal of neuroscience research, 77(2), 285-291 (2004-06-24)
Uncoupling proteins (UCPs) have been reported to decrease the mitochondrial production of reactive oxygen species (ROS) by lowering the mitochondrial inner membrane potential (MMP). We have previously shown that UCP3 expression is positively regulated by insulin-like growth factor-1 (IGF-1). The
Xiaoyan Sun et al.
American journal of physiology. Endocrinology and metabolism, 285(3), E512-E520 (2003-05-02)
Uncoupling protein 3 (UCP3) is a member of the mitochondrial transporter superfamily that is expressed primarily in skeletal muscle. UCP3 is upregulated in various conditions characterized by skeletal muscle atrophy, including hyperthyroidism, fasting, denervation, diabetes, cancer, lipopolysaccharide (LPS), and treatment
M Mogensen et al.
The Journal of physiology, 571(Pt 3), 669-681 (2006-01-21)
The purpose of this study was to investigate the hypothesis that cycling efficiency in vivo is related to mitochondrial efficiency measured in vitro and to investigate the effect of training status on these parameters. Nine endurance trained and nine untrained
Anne D Hafstad et al.
Cardiovascular research, 83(3), 519-526 (2009-04-29)
Myocardial fatty acid (FA) oxidation is regulated acutely by the FA supply and chronically at the transcriptional level owing to FA activation of peroxisome proliferator-activated receptor-alpha (PPARalpha). However, in vivo administration of PPARalpha ligands has not been shown to increase
K Sahlin et al.
American journal of physiology. Endocrinology and metabolism, 292(1), E223-E230 (2006-08-24)
The purpose of this study was to investigate fatty acid (FA) oxidation in isolated mitochondrial vesicles (mit) and its relation to training status, fiber type composition, and whole body FA oxidation. Trained (Vo(2 peak) 60.7 +/- 1.6, n = 8)
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