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324626

Millipore

EGTA, Molecular Biology Grade

EGTA, Molecular Biology Grade

Synonyme(s) :

EGTA, Molecular Biology Grade, Ethyleneglycol- bis(β-aminoethyl)-N,N,Nʹ,Nʹ-tetraacetic Acid

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25 G
186,00 €

186,00 €


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25 G
186,00 €

About This Item

Formule empirique (notation de Hill):
C14H24N2O10
Numéro CAS:
Poids moléculaire :
380.35
Numéro MDL:
Code UNSPSC :
12352106
Nomenclature NACRES :
NA.25

186,00 €


En stockDétails


Devis pour commande en gros

Qualité

Molecular Biology

Niveau de qualité

Essai

≥98% dry basis (complexometric)

Forme

solid (fine)

Pertinence de la réaction

reagent type: chelator

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

OK to freeze

Couleur

white

Solubilité

1 M NaOH: soluble

Traces de cations

heavy metals (as Pb): ≤0.001%

Activité étrangère

DNases, proteases, RNases, none detected

Conditions d'expédition

ambient

Température de stockage

10-30°C

Chaîne SMILES 

N(CCOCCOCCN(CC(=O)O)CC(=O)O)(CC(=O)O)CC(=O)O

InChI

1S/C14H24N2O10/c17-11(18)7-15(8-12(19)20)1-3-25-5-6-26-4-2-16(9-13(21)22)10-14(23)24/h1-10H2,(H,17,18)(H,19,20)(H,21,22)(H,23,24)

Clé InChI

DEFVIWRASFVYLL-UHFFFAOYSA-N

Description générale

Chelating agent selective for Ca2+. Binds one cation per molecule. Induces apoptosis in pheochromocytoma (PC12) cells.

Application


  • Live imaging of intra-lysosome pH in cell lines and primary neuronal culture using a novel genetically encoded biosensor.: This study presents a genetically encoded biosensor for real-time monitoring of lysosomal pH changes, potentially enhanced by buffering agents like EGTA. The findings may offer insights into cellular mechanisms influenced by calcium chelation (Ponsford et al., 2021).

  • Comparative Proteomic Analysis of Pleurotus ostreatus Reveals Great Metabolic Differences in the Cap and Stipe Development and the Potential Role of Ca(2+).: The research highlights the role of calcium in fungal development, with EGTA potentially influencing these biological processes through calcium chelation (Zhu et al., 2019).


Avertissement

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 1 year at 4°C.

Autres remarques

Lindenboim, L., et al. 1995. J. Neurochem.64, 1054.
Bers, D.M. 1982. Am. J. Physiol.242, C404.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 2


Certificats d'analyse (COA)

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Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

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