234109-M

Colcemid

Cell synchronization agent.

• Synonyme(s) : Colcemid, Demecolcine
• Formule empirique (notation de Hill): C₂₁H₂₅NO₅
• Numéro CAS: 477-30-5
• Poids moléculaire : 371.43
• Numéro MDL: MFCD00075459
• Code UNSPSC : 12352200

Propriétés

• Pureté: ≥98% (HPLC)
• Forme: crystalline powder
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze
• Solubilité: chloroform: 10 mg/mL; ethanol: soluble
• Température de stockage: 10-30°C
• InChI: 1S/C21H25NO5/c1-22-15-8-6-12-10-18(25-3)20(26-4)21(27-5)19(12)13-7-9-17(24-2)16(23)11-14(13)15/h7,9-11,15,22H,6,8H2,1-5H3
• Clé InChI: NNJPGOLRFBJNIW-UHFFFAOYSA-N

Description

Description générale

Cell synchronization agent. Induces apoptosis by blocking mitosis in HeLa S3 cells. Depolymerizes microtubules and limits microtubule formation. Appropriate dilutions inactivate spindle fiber mechanism during metaphase, systematically immobilizing chromosomes at the metaphase plate.

Cell synchronization agent. Induces apoptosis by blocking mitosis in HeLa S3 cells. Depolymerizes microtubules and limits microtubule formation. Appropriate dilutions inactivate the spindle fiber mechanism during metaphase, systematically immobilizing chromosomes at the metaphase plate.

Actions biochimiques/physiologiques

Primary Target
Depolymerizes microtubules and limits microtubule formation.

Avertissement

Toxicity: Toxic & Carcinogenic / Teratogenic (G)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) under an inert gas. Stock solutions are stable for up to 6 months at -20°C.

Autres remarques

Urbani, L., et al. 1995. Exp. Cell Res. 219, 159.
Kanda, R., et al. 1994. J. Radiat. Res. 35, 41.
Sherwood, S.W., et al. 1994. Exp. Cell Res. 215, 373.
Nishiyama, I., and Fuji, T. 1992. Exp. Cell Res.198, 214.
Spoerri, P.E., and Roisen, F.J. 1992. J. Neurosci. Res.31, 494.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany