218807

Caspase-9, Human, Recombinant, E. coli

Caspase-9, Human, Recombinant is expressed in E. coli as a single polypeptide. Fused at the N- and C- termini to a His•Tag sequence. Undergoes spontaneous autoprocessing to yield the active enzyme.

• Synonyme(s) : ICE-LAP6, Mch6
• Code UNSPSC : 12352202

Propriétés

• Produit recombinant: expressed in E. coli
• Niveau de qualité: 100
• Pureté: ≥70% (SDS-PAGE)
• Forme: lyophilized
• Activité spécifique: ≥400 units/mg protein
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze
• Solubilité: PBS with 15% glycerol: 1 units/μL
• Conditions d'expédition: wet ice
• Température de stockage: −70°C

Description

Description générale

Recombinant, human caspase-9 fused at the N- and C- termini to a His•Tag sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. MW: 11000 and 20000.

Avertissement

Toxicity: Standard Handling (A)

Définition de l'unité

One unit is defined as the amount of enzyme that will cleave 1.0 nmol substrate Ac-LEHD-pNA (Cat. No. 218805) per h at 37°C, pH 7.2.

Forme physique

Lyophilized from 130 mM NaCl, 20 mM Tris, 4 mM (NH₄)₂SO₄

Reconstitution

Following reconstitution, aliquot and freeze (-70°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 4 months at -20°C.

Autres remarques

Qin, H., et al. 1999. Nature399, 549.
Li, P., et al. 1997. Cell91, 479.
Salvesen, G.S., et al. 1997. Cell91, 443.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1