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Merck
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17-500M

Millipore

Catch and Release v2.0 Reversible Immunoprecipitation System

Synonyme(s) :

Catch and release system, Immunoprecipitation kit

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About This Item

Code UNSPSC :
12161503
eCl@ss :
32161000
Nomenclature NACRES :
NB.22

Réactivité de l'espèce (prédite par homologie)

all

Niveau de qualité

100

Fabricant/nom de marque

Catch And Release
Upstate®

Technique(s)

activity assay: suitable (IP-kinase)
immunoprecipitation (IP): suitable
western blot: suitable

Conditions d'expédition

wet ice

Description générale

Features & Benefits:
Reproducible: eliminates aspiration steps that typically lead to diminished/lost sample
Convenient: spin-column format dramatically simplifies sample handling
Reversible: simple elution of antigen:antibody complex in either native or denatured form



Use:
This kit allows for quick and reproducible immunoprecipitation (IP) by using a spin column. The system is more reproducible than regular IP′s, which are problematic with regards to washing the protein A/G agarose without disrupting the agarose bed. The binding of the antibody/antigen complex in Catch and Release is reversible, and elution of the immune complex can occur with native or denaturing buffers. The system has been tested successfully with rabbit, mouse, sheep and goat antibodies. IP using human IgG1-4 should be suitable. IP using chicken antibodies or human IgA, IgD, IgE or IgM is not recommended with this kit. Please read the enclosed product manual before use.

Application

Catch and Release v2.0 should not be used with proteins containing a 6-His tag.

Conditionnement

50 immunoprecipitations

Composants

Catch and Release Wash Buffer, 10X (Cat.# 20-210)

Catch and Release Non-denaturing Elution Buffer, 4X (Cat.# 20-209)

Catch and Release Denaturing Elution Buffer, 1X (Cat.# 20-284)

20-285

20-216

Catch and Release Capture Tubes

Qualité

routinely evaluated by immunoprecipitation.

Stockage et stabilité

All components to be stored at 4°C except the Catch and Release Capture Tubes which are stored at room temperature. Components are stable for 6 months from date of shipment.

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogrammes

Health hazardCorrosionEnvironment
GHS08,GHS05,GHS09

Mention d'avertissement

Danger

Mentions de danger

H314,H360D,H410

Classification des risques

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Repr. 1B - Skin Corr. 1C

Code de la classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Wanxia He et al.
Journal of virology, 77(19), 10566-10574 (2003-09-13)
Addition of human papillomavirus (HPV) E7 CDK2/cyclin A or CDK2/cyclin E, purified from either insect cells or bacteria, dramatically upregulates histone H1 kinase activity. Activation is substrate specific, with a smaller effect noted for retinoblastoma protein (Rb). The CDK2 stimulatory
Hypercholesterolemia-induced Abeta accumulation in rabbit brain is associated with alteration in IGF-1 signaling.
Sharma, Sunita, et al.
Neurobiology of Disease, 32, 426-432 (2008)
Enhanced protein recovery and reproducibility from pull-down assays and immunoprecipitations using spin columns.
A Brymora et al.
Analytical biochemistry, 295(1), 119-122 (2001-07-31)
E-Y Huang et al.
Cell death & disease, 3, e251-e251 (2012-01-13)
Galectin-1 is a lectin recognized by galactoside-containing glycoproteins, and is involved in cancer progression and metastasis. The role of galectin-1 in radiosensitivity has not previously been investigated. Therefore, this study tests whether galectin-1 is involved in the radiosensitivity mediated by
INFLUENCE OF GLUCOSAMINE ON GLOMERULAR MESEANGIAL CELL TURNOVER: IMPLICATIONS FOR HYPERGLYCEMIA AND HEXOSAMINE PATHWAY FLUX.
James LR, Le C, Scholey JW
American Journal of Physiology. Endocrinology and Metabolism null
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