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14-326-M

Sigma-Aldrich

Src Protein, active, 10 µg

Active, N-Terminal His6-tagged, recombinant, full-length, human Src. For use in Kinase Assays.

Synonyme(s) :

SRC1

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About This Item

Code UNSPSC :
12352202
eCl@ss :
32160405
Nomenclature NACRES :
NA.26

Source biologique

human

Niveau de qualité

Produit recombinant

expressed in Sf21 cells

Forme

liquid

Poids mol.

Mw 61.7 kDa

Fabricant/nom de marque

Upstate®

Technique(s)

activity assay: suitable (kinase)

Solubilité

soluble

Numéro d'accès NCBI

Numéro d'accès UniProt

Informations sur le gène

human ... SRC(6714)

Description générale

Research area: Cell Signaling

N-Terminal His6-tagged, recombinant, full-length, human Src.

Src proteins are non-receptor protein tyrosine kinases, that belong to the Src family of kinases. These proteins have a unique structure consisting of an N-terminal region with a 14-carbon myristoyl group, two Src homology domains (SH2 and SH3), a catalytic tyrosine-protein kinase domain (SH1), and a short C-terminal tail. The SH1 domain is catalytic in function, while the SH2 and SH3 domains are non-catalytic and regulatory in nature.

Product Source: Human c-src, expressed in Sf21 cells.

Application

The Src protein may be used to study the interaction between CD133 and Src protein and its effect on FAK phosphorylation and cell migration through in vitro kinase assay.

Actions biochimiques/physiologiques

Src protein tyrosine kinases are involved in signaling pathways that control a plethora of cellular functions ranging from cell cycle, proliferation, differentiation, gene transcription, immune responses, apoptosis, etc. Upon its activation, Src phosphorylates and activates several other kinases Like MAPK (Mitogen-Activated Protein Kinase), p38, and ERK (Extracellular signal-regulated kinase). Activated MAPK (Mitogen-Activated Protein Kinase) regulates inflammation, cell development, differentiation and senescence. P38 is involved with the migration and survival of endothelial cells and extracellular signal-regulated kinase (ERK) promotes the proliferation and inflammation of endothelial cells. They play a key role in the regulation of cytoskeletal organization through the phosphorylation of substrates like Actin filament-associated protein 1 (AFAP1) and cortactin (CTTN). Src also plays a crucial role in maintaining cell adhesion, morphology, motility, and bone resorption by interacting with focal adhesion kinases. Src tyrosine kinase activity is upregulated in a variety of cancers including colon, lung, and pancreatic cancer, etc.

Conditionnement

2 vials at 5μg each
Also available in 250μg size (2x125μg)--call for pricing and availability and reference catalog number 14-326M when ordering the 250μg size.

Qualité

Routinely evaluated by phosphorylation of Src Substrate Peptide

Autres remarques

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogrammes

Exclamation mark

Mention d'avertissement

Warning

Mentions de danger

Classification des risques

Skin Sens. 1

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Jianhui Ma et al.
Cancer cell, 35(3), 504-518 (2019-03-05)
Ionizing radiation (IR) and chemotherapy are standard-of-care treatments for glioblastoma (GBM) patients and both result in DNA damage, however, the clinical efficacy is limited due to therapeutic resistance. We identified a mechanism of such resistance mediated by phosphorylation of PTEN
Protein tyrosine kinases, with emphasis on the Src family.
Courtneidge, S A
Seminars in Cancer Biology, 5, 239-246 (1994)

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