1.32647
Alcian blue staining kit, pH 1.0
for the detection of strongly sulfated mucins for microscopy
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Produits recommandés
Niveau de qualité
IVD
for in vitro diagnostic use
pH
1.0
Application(s)
clinical testing
diagnostic assay manufacturing
hematology
histology
Température de stockage
15-25°C
Description générale
The ready-to-use Alcian blue stainig kit, pH 1.0 - for the detection of strongly sulfated mucins - for microscopy, can be used for this staining in histological tissue. Alcian blue pH 1.0 is used to stain strongly sulfated mucins in human-histological specimen materials, for example histological sections of e. g. colon or bronchus. The dye Alcian blue 8 GX (Product number 1.01647) is a water-soluble phthalocyanine dye. Acidic mucins have a negative charge and bind the positively charged Alcian blue 8 GX staining dye.
A solution of hydrochloric acid and Alcian blue is used for the staining procedure. At a strongly acidic pH of 1.0, this solution suppresses the dissociation of the carboxyl groups and selectively stains only the strongly sulfated mucins and polyanions. Counterstaining takes place with a nuclear fast red/aluminium sulfate solution. The undifferentiated method (Alcian blue 8 GX solution, pH 2.5) enables only the detection of acidic glycoproteins, e. g. mucins, without distinguishing between carboxyl and sulfate groups.
The package is sufficient for approx. 2500 applications. For more details, please see instructions for use (IFU). This product is registered as IVD and CE marked.
A solution of hydrochloric acid and Alcian blue is used for the staining procedure. At a strongly acidic pH of 1.0, this solution suppresses the dissociation of the carboxyl groups and selectively stains only the strongly sulfated mucins and polyanions. Counterstaining takes place with a nuclear fast red/aluminium sulfate solution. The undifferentiated method (Alcian blue 8 GX solution, pH 2.5) enables only the detection of acidic glycoproteins, e. g. mucins, without distinguishing between carboxyl and sulfate groups.
The package is sufficient for approx. 2500 applications. For more details, please see instructions for use (IFU). This product is registered as IVD and CE marked.
Application
For the detection of strongly sulfated mucins.
Remarque sur l'analyse
Nuclei: red
Strongly sulfated mucins: light blue
Strongly sulfated mucins: light blue
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Eye Dam. 1 - Met. Corr. 1
Code de la classe de stockage
8A - Combustible, corrosive hazardous materials
Classe de danger pour l'eau (WGK)
WGK 2
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Active Filters
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique
