05-1076

Anti-c-Jun Antibody, clone 6E4.4

clone 6E4.4, from mouse

• Synonyme(s) : JNK1 alpha protein kinase, JNK1 beta protein kinase, JUN N-terminal kinase, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1, mitogen-activated protein kinase 8, mitogen-activated protein kinase 8 isoform JNK1 alpha1, mitogen-activated pro
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 6E4.4, monoclonal
• Espèces réactives: mouse, human, rat
• Conditionnement: antibody small pack of 25 μg
• Technique(s): immunocytochemistry: suitable; western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_002219.1
• Numéro d'accès UniProt: P45983
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... JUN(3725) ; mouse ... Jun(16476) ; rat ... Jun(24516)

Description

Description générale

c-Jun is a component of the transcription factor AP-1 that binds and activates transcription at TRE/AP-1 elements and appears to be a major downstream target of the SAPK/JNK signaling pathway. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73. Extracellular signals including growth factors, transforming oncoproteins and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun dependent transcription. Mutation of Ser63/73 renders c-Jun nonresponsive to mitogenic and stress induced signaling pathways. The MAP kinase homologue, SAPK/JNK, binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. In addition, the activity of SAPK/JNK is stimulated by the same signals that activate c-Jun.

Spécificité

This antibody detects c-Jun, regardless of phosphorylation state.

Immunogène

GST-tagged recombinant protein corresponding to c-Jun.

Application

Anti-c-Jun Antibody, clone 6E4.4 is a Mouse Monoclonal Antibody for detection of c-Jun also known as JNK1 alpha protein kinase, JNK1 beta protein kinase & has been validated in WB, ICC.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Western Blot Analysis (SNAP ID Technique): 5 µg/mL of this antibody detected c-Jun on 15 µg of NIH/3T3 cell lysate using the SNAP ID Tecnique).

Immunocytochemistry Analysis: Confocal fluorescent analysis of HeLa, C2C12, and A431 cells using anti-c-Jun, clone 6E4.4 mouse monoclonal antibody (see page 2).

Qualité

Evaluated by Western Blot in NIH/3T3 cell lysate.

Western Blot Analysis: 0.5-2 µg/mL of this antibody detected c-Jun on 15 µg of NIH/3T3 cell lysate.

Description de la cible

~36-43 kDa
Multiple bands may be visible, indicating various phosphorylation states.

Liaison

Replaces: 04-210

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable at 2-8°C for 1 year from date of receipt.

Remarque sur l'analyse

Control
NIH/3T3 cell lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable