SEP020 is designed to deplete the top 14 abundant human proteins, while PROT20LC is designed to deplete the top 20 abundant human proteins. Also, all of the antibodies in SEP020 are IgY antibodies, while the PROT20LC antibodies and antibody ligands are primarily IgG-based.
Recommended Products
column D × L
6.4 mm × 63.0 mm
capacity
2.5 mg total protein loading(35 μL of human plasma, average human protein concentration 70 mg/mL)
storage temp.
2-8°C
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General description
The targeted highly abundant proteins are simultaneously removed by the immobilized specific IgYs when crude biological samples are passed through the column. Selective immunodepletion provides an enriched pool of low abundance proteins for downstream proteomic analyses. Specific removal of these 14 highly abundant proteins depletes ~95% of the total protein mass from human serum or plasma. The low abundance proteins in the flow-through fractions can then be studied. Removal of highly abundant proteins enables improved resolution and dynamic range for one dimensional electrophoresis (1DGE), two-dimensional (2DGE) electrophoresis and liquid chromatography/ mass spectrometry (LC/MS). The collected flow-through fractions may need to be concentrated dependent upon the downstream application.
Seppro® IgY14 columns allow users to separate 14 highly abundant proteins (HAP) from plasma protein mixtures. The Avian polyclonal IgY (Immunoglobulin Yolk) antibodies, have a distinct, highly specific partitioning advantage over competing products, allowing the researcher to look at a much cleaner human protein mixture. Seppro® columns make further proteomics analysis into the plasma proteome much easier and provide cleaner results.
Proteins Depleted:
Albumin
IgG
α1-Antitrypsin
IgA
IgM
Transferrin
Haptoglobin
α2-Macroglobulin
Fibrinogen
Complement C3
α1-Acid Glycoprotein (Orosomucoid)
HDL (Apolipoproteins A-I and A-II)
LDL (mainly Apolipoprotein B)
Proteins Depleted:
Albumin
IgG
α1-Antitrypsin
IgA
IgM
Transferrin
Haptoglobin
α2-Macroglobulin
Fibrinogen
Complement C3
α1-Acid Glycoprotein (Orosomucoid)
HDL (Apolipoproteins A-I and A-II)
LDL (mainly Apolipoprotein B)
Application
Seppro® IgY14, LC2 (2 mL bed volume columns) is designed for use as a plasma/serum protein removal tool base upon immune affinity removal of 14 highly abundant proteins (HAP) from human plasma protein mixtures via liquid chromatography. Seppro® IgY14 is used to support the analysis of low abundance human serum/plasma proteins.
Storage and Stability
Store the column at 2–8 °C. After use, equilibrate the column with 1´ Dilution Buffer containing 0.02% sodium azide and store the column at 2–8 °C with the end-caps tightly sealed. Do Not Freeze the column.
Other Notes
If the protein concentration of the sample is unknown and the total serum protein level is potentially elevated, a reduction of the serum load to 24.0 ml is recommended for initial study to avoid potential abundant protein bleed through.
Legal Information
Seppro is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Effect of plasma protein depletion on BNP-32 recovery.
Adam M Hawkridge et al.
Clinical chemistry, 54(5), 933-934 (2008-04-30)
Does plasma protein depletion increase lung liquid conductance?
P Dodek
American journal of respiratory and critical care medicine, 154(4 Pt 1), 1213-1215 (1996-10-01)
Renato Millioni et al.
PloS one, 6(5), e19603-e19603 (2011-05-17)
To date, the complexity of the plasma proteome exceeds the analytical capacity of conventional approaches to isolate lower abundance proteins that may prove to be informative biomarkers. Only complex multistep separation strategies have been able to detect a substantial number
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How would I decide between Products SEP020, Seppro® IgY, and PROT20LC, ProteoPrep® 20 Plasma Immunodepletion LC Columns?
1 answer-
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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My protein sample contains SDS. Is this compatible with Product SEP020, Seppro® IgY14?
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No. We do not recommend using protein samples containing SDS with Product No. SEP020. SDS is a detergent and would be expected to affect adversely the activity of the conjugated antibody in terms of depletion.
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How does the storage temperature relate to shipping conditions?
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The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
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