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SAB4200651

Sigma-Aldrich

Monoclonal Anti-Histone H3 (C-terminal) antibody produced in mouse

clone H6.10, purified from hybridoma cell culture

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

H6.10, monoclonal

form

buffered aqueous solution

mol wt

antigen ~14 kDa

species reactivity

chicken, human, monkey, hamster, canine

concentration

~1 mg/mL

technique(s)

immunoblotting: 0.25-0.5 μg/mL using using histones isolated from human HeLa cells.
immunofluorescence: 5-10 μg/mL using using HeLa cells.

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... HIST1H3A(8350)

General description

Histone cluster 1 H3A (HIST1H3A) belongs to the H3 histone family. The gene encoding it is localized on human chromosome 6.
Monoclonal Anti-Histone H3 (C-terminal) (mouse IgG1 isotype) is derived from the hybridoma H6.10 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a sequence at the C terminal region of human Histone H3, conjugated to KLH.

Immunogen

a synthetic peptide corresponding to a sequence at the C terminal region of human Histone H3.

Application

Monoclonal Anti-Histone H3 (C-terminal) antibody has been used in
  • immunoblotting
  • immunofluorescence
  • western blotting

Biochem/physiol Actions

H3.1 (Histone 3-variant 1) is involved in gene silencing as well as gene activation. Histones H3 and H4 are the predominant histones subjected to extensive covalent modifications. Mutations in histone H3 were identified in various tumors.
Histone cluster 1 H3A (HIST1H3A) has been associated with DNA replication.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Expression patterns and post-translational modifications associated with mammalian histone H3 variants
Hake S B, et al.
The Journal of Biological Chemistry, 281(1), 559-568 (2006)
Histone H3. 3 mutations: a variant path to cancer
Yuen B T K and Knoepfler P S
Cancer Cell, 24(5), 567-574 (2013)
Sean M Callahan et al.
Science advances, 9(32), eade2693-eade2693 (2023-08-11)
Histone modifications control numerous processes in eukaryotes, including inflammation. Some bacterial pathogens alter the activity or expression of host-derived factors, including sirtuins, to modify histones and induce responses that promote infection. In this study, we identified a deacetylase encoded by
FERONIA receptor kinase contributes to plant immunity by suppressing jasmonic acid signaling in Arabidopsis thaliana
Guo H, et al
Current Biology, 28(20), 3316-3324 (2018)
Organismal differences in post-translational modifications in histones H3 and H4
Garcia B, et al.
The Journal of Biological Chemistry, 282(10), 7641-7655 (2007)
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