G8795
Anti-GAPDH antibody, Mouse monoclonal
clone GAPDH-71.1, purified from hybridoma cell culture
Synonym(s):
GAPDH Antibody - Monoclonal Anti-GAPDH antibody produced in mouse, Gapdh Antibody, Anti-G3PD, Anti-G3PDH, Anti-Glyceraldehyde-3-phosphate dehydrogenase, Loading Control
Sign Into View Organizational & Contract Pricing
All Photos(6)
About This Item
Recommended Products
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
purified immunoglobulin
antibody product type
primary antibodies
clone
GAPDH-71.1, monoclonal
form
buffered aqueous solution
mol wt
antigen ~37 kDa
species reactivity
bovine, turkey, canine, chicken, monkey, mink, mouse, human, rabbit, rat, hamster
should not react with
prokaryotes
packaging
antibody small pack of 25 μL
concentration
~1 mg/mL
technique(s)
immunocytochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.025-0.05 μg/mL using A431 total cell extract
isotype
IgM
UniProt accession no.
application(s)
research pathology
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... GAPDH(2597)
mouse ... Gapdh(14433)
rat ... Gapdh(24383)
General description
Monoclonal Anti-GAPDH (mouse IgM isotype) is derived from the hybridoma GAPDH-71.1 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with rabbit GAPDH (glyceraldehyde-3-phosphate dehydrogenase). The gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is mapped to human chromosome 12p13. The protein localizes in the cytoplasm but can be translocated to the nucleus depending on cellular conditions.
Specificity
Monoclonal Anti-GAPDH recognizes human, monkey, bovine, canine, rat, mouse, hamster, mink, rabbit, chicken, and turkey GAPDH. It does not cross-react with non-vertebrate and prokaryotic species.
Immunogen
Rabbit GAPDH.
Application
Monoclonal Anti-GAPDH antibody produced in mouse is suitable for western blotting using:
- protein extracted from heart tissue of mice at a working dilution of 1:25,000
- myelin and axogliasomal fractions from human CNS
- nuclear and cytoplasmic fractions from TBP-13Q and TBP-105Q PC12 cells following recovery from heat shock
- protein from bovine immortalized luteal endothelial cells
- renal tubular epithelial cell extract
- proteins from mouse embryonic fibroblasts
- protein extract from ventricular myocardium tissues
- A431 total cell extract at a working concentration of 0.025-0.05μg/mL
Biochem/physiol Actions
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a tetramer containing identical chains. It catalyzes the reversible oxidative phosphorylation of glyceraldehyde-phosphate, which is a critical energy-yielding step in carbohydrate metabolism. It binds to several proteins including actin, tubulin, amyloid precursor, polyglutamine peptides, DRPLA (dentatorubral-pallidoluysian atrophy), and huntingtin. Protein kinase Cι/λbinds and phosphorylates GAPDH. Phosphorylated GAPDH associates with cytoskeletal elements and controls microtubule dynamics in the early secretory pathway. Poly(ADP-ribose) polymerase-1 (PARP1) interacts with GAPDH and thereby mediates brain damage in the presence of oxidative/nitrosative stress. GAPDH forms a part of OCA-S, the multicomponent OCT1 (octamer-motif-binding factor) coactivator complex, which is involved in the S phase-dependent histone H2B transcription. This association is responsible for linking H2B transcriptional machinery to cell cycle regulation and to the cellular metabolic state. GAPDH is also a component of the functional GAIT (interferon-γ-activated inhibitor of translation) mRNP (messenger ribonucleoprotein). GAPDH expression is dysregulated during melanoma progression.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month.
For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frostfree” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frostfree” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
recommended
Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Gloria Ravegnini et al.
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 26(5), 743-749 (2012-12-12)
Leiomyoma and leiomyosarcoma share morphological features and smooth muscle differentiation, and both arise most frequently within the uterine corpus of middle-aged women. However, they are considered biologically unrelated tumors due to their disparate clinical, cytogenetic, and molecular features. MED12, the
Frontiers in neurology and neuroscience research null
Xiaofeng Zuo et al.
The Journal of biological chemistry, 286(25), 22469-22477 (2011-05-06)
Primary cilia are found on many epithelial cell types, including renal tubular epithelial cells, where they participate in flow sensing. Disruption of cilia function has been linked to the pathogenesis of polycystic kidney disease. We demonstrated previously that the exocyst
Jean-Paul Decuypere et al.
PloS one, 8(4), e61020-e61020 (2013-04-09)
Autophagy is a lysosomal degradation pathway important for cellular homeostasis and survival. Inhibition of the mammalian target of rapamycin (mTOR) is the best known trigger for autophagy stimulation. In addition, intracellular Ca(2+) regulates autophagy, but its exact role remains ambiguous.
Hang Zhao et al.
American journal of physiology. Heart and circulatory physiology, 301(4), H1513-H1518 (2011-08-16)
Methionine sulfoxide reductase A (MsrA) catalytically scavenges reactive oxygen species and also repairs oxidized methionines in proteins. Increasing MsrA protects cells and organs from a variety of oxidative stresses while decreasing MsrA enhances damage, but the mechanisms of action have
Articles
Loading controls in western blotting application.
Warburg effect enhances glucose to lactate conversion in tumor cells, regardless of oxygen levels; impacting cancer metabolism since 1924.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service