MAK418
AMP Colorimetric Assay Kit
sufficient for 100 colorimetric tests
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About This Item
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detection method
colorimetric
relevant disease(s)
neurological disorders; aging/geriatric diseases; cancer
storage temp.
−20°C
General description
Adenosine Monophosphate (AMP), also known as 5′-adenylic acid, consists of adenine, ribose and phosphate. AMP can be produced during ATP synthesis by the enzyme adenylate kinase by combining two ADP molecules, as a result of the hydrolysis of ATP/ADP, or when RNA is broken down. AMP plays an important role in many cellular metabolic processes, such as Ca2+ signaling, cell migration, cytokine secretion etc. AMP is an activator of AMP-activated protein kinase, which regulates glucose uptake, fatty acid uptake, fatty acid oxidation etc. AMP levels can be measured by luciferase/luciferin mediated assays. However, luciferase signal is unstable and luminescent equipment is generally not available in most laboratories.
Features and Benefits
AMP Assay Kit:
- Compatible with high-throughput handling systems.
- Sensitive, detecting AMP levels lower than 10 μM in various sample types.
Suitability
The kit is suitable for the measurement of AMP levels in various cell (adherent or suspension cells: e.g. HeLa, HEK239, Jurkat cells) and tissue lysates (e.g. liver, kidney, etc.), the study of the regulation of AMPK by AMP and for the mechanistic study of key cellular processes such as Ca2+ signaling, glucose uptake, lipid uptake, etc.
Principle
In this assay, AMP is converted to pyruvate in the presence of pyrophosphate and phosphoenolpyruvate. This is followed by a set of enzymatic reactions to generate a colored product with a strong absorbance at A570 nm. The absorbance is proportional to the amount of AMP present in samples.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Repr. 1B - Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 3
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