08069

modified Tryptic Soy Broth- Dehydrated Culture Media

NutriSelect^® Plus, with bile salts mixture, according to ISO 16654:2001, powder, selective for enterovirulent E. coli (EEC) O157:H7

• Synonym(s): Modified Soyabean Bile Broth, Modified soybean bile broth base, Modified tryptone soy broth, Tryptic soy broth, modified, mTSB
• UNSPSC Code: 41171607
• NACRES: NA.85

Properties

• Agency: according to ISO 16654:2001
• Quality Level: 200
• sterility: non-sterile
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: casein enzymic hydrolysate, 17 g/L ; dipotassium hydrogen phosphate, 4 g/L ; D-glucose, 2.5 g/L ; papaic digest of soyabean meal, 3 g/L ; sodium chloride, 5 g/L ; with bile salts mixture
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbiological culture: suitable
• final pH: 7.3±0.2 (25 °C)
• application(s): food and beverages; microbiology; pathogen testing; vitamins, nutraceuticals, and natural products
• suitability: selective for enterovirulent E. coli (EEC) O157:H7

Description

Application

Modified tryptone soy broth is recommended as an enrichment medium for the detection of Escherichia coli O157:H7 from food and animal feeding stuffs.

Preparation Note

Suspend 33 grams of Modified tryptone soy broth in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 minutes. Cool to 45-50 °C and aseptically add rehydrated contents of 1 vial of VCC Selective Supplement (Cat. No. No. 80704). Mix well and dispense as desired.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable