AB3239-I
Anti-Myostatin Antibody, Near CT
from rabbit, purified by affinity chromatography
Synonym(s):
GDF8, Growth/differentiation factor 8, GDF-8, Myostatin
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
species reactivity (predicted by homology)
primate (based on 100% sequence homology), rat (based on 100% sequence homology), mouse (based on 100% sequence homology), monkey (based on 100% sequence homology), chimpanzee (based on 100% sequence homology)
technique(s)
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... MSTN(2660)
General description
Myostatin, also known as Growth/differentiation factor 8, is a negative regulator of skeletal muscle growth encoded by the MSTN (also known as MSLHP and GDF8) gene in human. Myostatin is a member of the bone morphogenetic protein (BMP) family within the TGF-ß superfamily. Growth and differentation factor-associated serum protein-1 & 2 (GASP-1/2) and follistatin-related gene (FLRG) protein are reported to interact with myostatin and negatively regulate its activity (PMID 24019467, 17878677). Mutation in MSTN gene is known to cause muscle hypertrophy (MSLHP), a condition characterized by increased muscle bulk and strength.
Immunogen
Epitope: Near C-terminal
KLH-conjugated linear peptide corresponding to human Myostatin near the C-terminal.
Application
Anti-Myostatin Antibody, Near CT is an antibody against Myostatin, Near CT for use in Western Blotting and Immunohistochemistry.
Immunohistochemistry Analysis: A 1:50-1,000 dilution from a representative lot detected Myostatin in human cardiac myocytes tissue.
Research Category
Signaling
Signaling
Research Sub Category
Signaling Neuroscience
Signaling Neuroscience
Quality
Evaluated by Western Blotting in human adult heart tissue lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Myostatin in 10 µg of human adult heart tissue lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Myostatin in 10 µg of human adult heart tissue lysate.
Target description
~31 kDa observed. 30–32 kDa band assumed to be a glycosylated dimer of the carboxy terminus portion of the full length translational product (Artaza, J.N., et al. (2007). J. of Endocrinology. 194:63-76). This antibody also detected the 32-kDa band corresponding to the processed dimer (Artaza, J.N., et al. (2007). J. of Endocrinology. 146(8):3547-3557).
Physical form
Affinity
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Alterations in myostatin expression are associated with changes in cardiac left ventricular mass but not ejection fraction in the mouse.
Artaza, JN; Reisz-Porszasz, S; Dow, JS; Kloner, RA; Tsao, J; Bhasin, S; Gonzalez-Cadavid, NF
The Journal of Endocrinology null
Myostatin inhibits myogenesis and promotes adipogenesis in C3H 10T(1/2) mesenchymal multipotent cells.
Artaza, JN; Bhasin, S; Magee, TR; Reisz-Porszasz, S; Shen, R; Groome, NP; Meerasahib et al.
Endocrinology null
Tamas Kocsis et al.
American journal of physiology. Endocrinology and metabolism, 312(3), E150-E160 (2016-12-15)
The TGFβ family member myostatin (growth/differentiation factor-8) is a negative regulator of skeletal muscle growth. The hypermuscular Compact mice carry the 12-bp Mstn(Cmpt-dl1Abc) deletion in the sequence encoding the propeptide region of the precursor promyostatin, and additional modifier genes of
Aniko Keller-Pinter et al.
FEBS letters, 592(18), 3139-3151 (2018-08-22)
Myostatin, a TGF-β superfamily member, is a negative regulator of muscle growth. Here we describe how myostatin activity is regulated by syndecan-4, a ubiquitous transmembrane heparan sulfate proteoglycan. During muscle regeneration the levels of both syndecan-4 and promyostatin decline gradually
Kay-Marie Lamar et al.
PLoS genetics, 12(5), e1006019-e1006019 (2016-05-07)
Latent TGFβ binding proteins (LTBPs) regulate the extracellular availability of latent TGFβ. LTBP4 was identified as a genetic modifier of muscular dystrophy in mice and humans. An in-frame insertion polymorphism in the murine Ltbp4 gene associates with partial protection against
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