The concentration of antibody in this ascitic fluid is typically in the range of 6 to 10 mg/mL.
I6510
Monoclonal Anti-Human IgE antibody produced in mouse
Monoclonal Anti-Human IgE antibody produced in mouse
1 of 1 reviewers received a sample product or took part in a promotion
clone GE-1, ascites fluid
Synonym(s):
Monoclonal Anti-Human IgE
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.46
Pricing and availability is not currently available.
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
secondary antibodies
clone
GE-1, monoclonal
contains
15 mM sodium azide
technique(s)
indirect ELISA: 1:5,000
isotype
IgG2b
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
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General description
Binds human IgE; does not bind human IgG, IgA or IgM.
Monoclonal Anti-Human IgE (mouse IgG2b isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulin E (IgE) antibodies are tetramers with two light chains (κ or λ) and two ε-heavy connected by numerous intrachain disulfide bonds. IgE exists in two isoforms, one is associated with the B cell receptor and the other being secreted by plasma cell. It has a short life span in plasma when compared to receptor bounded IgE.
Specificity
Monoclonal Anti-Human IgE is immunospecific for human IgE. No cross-reactivity with human IgG, IgM or purified light chains is observed.
Application
To confirm that a chimaeric monoclonal antibody had episolon chain, an elisa was performed using monoclonal anti-human IgE antibody (clone GE1) at a 1:1000 dilution.
Biochem/physiol Actions
Immunoglobulin E (IgE) is implicated in acute allergic reactions and chronic inflammatory allergic diseases, such as allergic asthma and chronic urticaria (CU). Receptor bounded IgE aids antigen uptake and modulates the differentiation and proliferation of B cells. It confers immunity against helminthic parasites. IgE is also involved in facilitating type I hypersensitivity reactions. It regulates mast cell homeostasis and also exhibits immunomodulatory effects.
Physical form
The product is provided as ascites fluid with 0.1% sodium azide as a preservative.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Analysis Note
The antibody is tested for reactivity against myeloma IgE and allergy-elevated IgE.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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