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A2672

Sigma-Aldrich

Monoclonal Anti-Albumin antibody produced in mouse

clone HSA-9, ascites fluid

Synonym(s):

Anti-AFP

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

HSA-9, monoclonal

contains

15 mM sodium azide

species reactivity

rhesus monkey, baboon, human

should not react with

catfish, turkey, chicken, hamster, rabbit, horse, donkey, cat, marmoset, pig, guinea pig, mouse, sheep, dog, pigeon, gibbon, bovine, goat

technique(s)

indirect ELISA: 1:500

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ALB(213)

General description

Albumin, the major protein produced by liver cells, represents more than half of the total protein content of human serum. Many other body fluids also contain albumin. Three major functions for serum albumin have been proposed: maintenance of osmotic pressure, transportation of a variety of substances and an endogenous source of amino acids. The primary sites of albumin degradation are not known, but the protein can be metabolized by almost every organ in the body. Determination of serum albumin levels is a widely used screening test in clinical medicine. A decrease in serum albumin levels may indicate disease states such as malnutrition, cirrhosis, nephrotic syndrome, diabetes, gastrointestinal and hepatic diseases, thermal burns and pulmonary disease.
Monoclonal anti-Human Serum Albumin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Specificity

The antibody is specific for human albumin in serum and plasma and recognizes an epitope sensitive to reduction commonly occurring in immunoblotting assays. No cross-reactivity is observed with bovine, cat, catfish, chicken, dog, donkey, gibbon, goat, guinea pig, hamster, horse, marmoset, mouse, pig, pigeon, rabbit, rat, sheep and turkey serum albumin.

Immunogen

Human platelets

Application

Monoclonal Anti-Albumin antibody produced in mouse has been used in:
  • enzyme linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunofluorescence

Western blot analysis of monkey retinal protein extracts were performed using the mouse monoclonal anti-albumin antibody at 1:20000 and overnight incubation at 4 degrees. Immunohistochemistry was performed on frozen monkey retina sections with the mouse monoclonal anti-albumin antibody at 1:1000. Uptake of albumin by LLC-PK1 cells was monitored by immunofluorescence using the mouse monoclonal anti-albumin antibody. Cells were first fixed in 4% formaldehyde the incubated with the antibody for 1 hour at room temperature.

Biochem/physiol Actions

Albumin is a transport protein that binds a broad range of ligands such as fatty acids, bilirubin, hemin, drugs, amino acids and ions . Albumin may also function as a zinc carrier protein and consequently regulate physiological processes . Three major functions for serum albumin have been proposed: maintenance of osmotic pressure, transportation of a variety of substances and an endogenous source of amino acids. The primary sites of albumin degradation are not known, but the protein can be metabolized by almost every organ in the body. Determination of serum albumin levels is a widely used screening test in clinical medicine. A decrease in serum albumin levels may indicate disease states such as malnutrition, cirrhosis, nephrotic syndrome, diabetes, gastrointestinal and hepatic diseases, thermal burns and pulmonary disease.
Serum albumins are implicated in the three major functions such as maintenance of osmotic pressure, transportation of a variety of substances and an endogenous source of amino acids. The primary sites of albumin degradation are not known, but the protein can be metabolized by almost every organ in the body. Determination of serum albumin levels is a widely used screening test in clinical medicine. A decrease in serum albumin levels may indicate disease states such as malnutrition, cirrhosis, nephrotic syndrome, diabetes, gastrointestinal and hepatic diseases, thermal burns and pulmonary disease.

Physical form

The product is provided as ascites fluid with 0.1% sodium azide as a preservative.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Hypoalbuminemia: pathogenesis and clinical significance
Soeters PB, et al.
Journal of Parenteral and Enteral Nutrition, 43(2), 181-193 (2019)
Overview of albumin and its purification methods
Raoufinia R, et al.
Advanced Pharmaceutical Bulletin, 6(4), 495-495 (2016)
Carlo Giannini et al.
Hepatology (Baltimore, Md.), 38(1), 114-122 (2003-06-28)
Allogenic hepatocyte transplantation or autologous transplantation of genetically modified hepatocytes has been used successfully to correct congenital or acquired liver diseases and can be considered as an alternative to orthotopic liver transplantation. However, hepatocytes are neither easily maintained in culture
Modulation of human mesenchymal stem cell immunogenicity through forced expression of human cytomegalovirus us proteins
Soland MA, et al.
PLoS ONE, 7(5), e36163-e36163 (2012)
Fadi-Luc Jaber et al.
The American journal of pathology, 193(1), 27-38 (2022-10-30)
Inadequate DNA damage response related to ataxia telangiectasia mutated gene restricts hepatic regeneration in acute liver failure. Resolving mechanistic gaps in liver damage and repair requires additional animal models that are unconstrained by ultrarapid and unpredictable mortalities or substantial divergences

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