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A6075

Sigma-Aldrich

Monoclonal Anti-Chicken Egg Albumin (Ovalbumin) antibody produced in mouse

clone OVA-14, ascites fluid

Synonym(s):

Anti-Albumin, chicken egg

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About This Item

MDL number:
MFCD00162221
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

OVA-14, monoclonal

mol wt

antigen 45 kDa

contains

15 mM sodium azide

species reactivity

chicken, turkey

should not react with

mouse, bovine, horse, canine, feline, pig, pigeon, rabbit, guinea pig, goat, sheep, donkey, rat, human

technique(s)

dot blot: suitable
indirect ELISA: 1:10,000 using chicken egg albumin (ovalbumin)
western blot: suitable

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

chicken ... Alb(396197)

General description

Monoclonal Anti-Chicken Egg Albumin (Ovalbumin) (mouse IgG1 isotype) is produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Immunogen

chicken egg albumin.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Monoclonal Anti-Chicken Egg Albumin (Ovalbumin) antibody produced in mouse.

Biochem/physiol Actions

Ovalbumin (OVA) is the main component of egg white proteins in hens. OVA stimulates IgE-mediated hypersensitivity responses in individuals with egg allergy. The protein has also been used to study structure-function relationships in allergens. Thus, Monoclonal Anti-Chicken Egg Albumin (Ovalbumin) antibody can be a useful tool for the analysis of egg albumin function in mediating food allergies.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Oliver Schulz et al.
Nature, 433(7028), 887-892 (2005-02-16)
Cross-presentation of cell-associated antigens plays an important role in regulating CD8+ T cell responses to proteins that are not expressed by antigen-presenting cells (APCs). Dendritic cells are the principal cross-presenting APCs in vivo and much progress has been made in
Chenjie Yang et al.
PloS one, 6(8), e22517-e22517 (2011-08-11)
Exosomes are endosome-derived small membrane vesicles that are secreted by most cell types including tumor cells. Tumor-derived exosomes usually contain tumor antigens and have been used as a source of tumor antigens to stimulate anti-tumor immune responses. However, many reports
Abdul Mannan Baru et al.
European journal of immunology, 40(8), 2259-2266 (2010-06-15)
Recent studies highlight the role of Treg in preventing unnecessary responses to allergens and maintaining functional immune tolerance in the lung. We investigated the role of Treg during the sensitization phase in a murine model of experimental allergic airway inflammation
Christina Hesse et al.
European journal of immunology, 43(10), 2543-2553 (2013-06-21)
Vaccination is one of the oldest yet still most effective methods to prevent infectious diseases. However, eradication of intracellular pathogens and treatment of certain diseases like cancer requiring efficient cytotoxic immune responses remain a medical challenge. In mice, a successful
H Kahlert et al.
Molecular immunology, 29(10), 1191-1201 (1992-10-01)
Ovalbumin (OVA) is a major allergen (Gal d II) of hen egg white and is often the cause of hypersensitivity reactions to food. Further knowledge of the antigenic and allergenic epitopes of allergens will provide better treatment of this disease.
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