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MAK046

Sigma-Aldrich

Lipase Activity Assay Kit

sufficient for 100 colorimetric tests (with glycerol standard)

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.

usage

sufficient for 100 colorimetric tests (with glycerol standard)

detection method

colorimetric

relevant disease(s)

pulmonary disorders; gastrointestinal diseases; cancer

storage temp.

−20°C

General description

The lipase family of enzymes catalyze the cleavage of the ester bonds of lipids. In mammals, this family includes many critical members including pancreatic, hepatic, endothelial, and lipoprotein lipase. Lipases, such as pancreatic lipase, are critical for the metabolism of lipids. Lipases also play key roles in processes such as cell signaling and inflammation. Measurements of lipase activity are commonly used to screen for pancreatic injury or disease, and to monitor diseases such as cystic fibrosis, celiac disease, and Crohn′s disease.

Application

Lipase Activity Assay Kit has been used to determine the lipase activity in samples.[1][2]

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the detection of lipase activity in biological samples including tissue, cells and serum

Principle

The Lipase Activity Assay kit provides a simple and direct procedure for measuring lipase activity in a variety of samples. Lipase activity is determined using a coupled enzyme reaction, which results in a colorimetric (570 nm) product proportional to the enzymatic activity present. One unit of Lipase is the amount of enzyme that will generate 1.0 μmole of glycerol from triglycerides per minute at 37 °C.

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Signal Word

Danger

Hazard Statements

H315,H317,H318,H334,H335

Hazard Classifications

Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

10 - Combustible liquids

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
8K27K07220
8H15K07220
8D13K07220
8D12K07220
7L12K07220

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Kristina Martinez-Guryn et al.
Cell host & microbe, 23(4), 458-469 (2018-04-13)
The gut microbiota play important roles in lipid metabolism and absorption. However, the contribution of the small bowel microbiota of mammals to these diet-microbe interactions remains unclear. We determine that germ-free (GF) mice are resistant to diet-induced obesity and malabsorb
Amélie de Vallée et al.
Frontiers in microbiology, 10, 2829-2829 (2019-12-24)
The gray mold fungus Botrytis cinerea is a necrotrophic pathogen able to infect hundreds of host plants, including high-value crops such as grapevine, strawberry and tomato. In order to decipher its infectious strategy, a library of 2,144 mutants was generated
Effect of phenolic hydroxyl groups on inhibitory activities of phenylpropanoid glycosides against lipase.
Wu X, et al.
Journal of functional foods, 38, 510-518 (2017)
Jeong Kuk Park et al.
IUCrJ, 6(Pt 4), 729-739 (2019-07-19)
Ependymin was first discovered as a predominant protein in brain extracellular fluid in fish and was suggested to be involved in functions mostly related to learning and memory. Orthologous proteins to ependymin called ependymin-related proteins (EPDRs) have been found to
Enhanced absorption of long-chain polyunsaturated fatty acids following consumption of functional milk formula, pre-digested with immobilized lipase ex vivo, in an exocrine pancreatic insufficient (EPI) pig model.
Goncharova K, et al.
Journal of functional foods, 34, 422-430 (2017)
View All Related Papers

Questions

1–10 of 10 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. What is the LOD/LOQ for this kit?

    1 answer

    1. This lipase assay kit detects lipase activity as low as 0.02 mU per well.

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  4. Are there available applications for running MAK046, MAK047, and MAK048 on Beckman chemistry analyzers like the AU5800 and AU680?

    1 answer

    1. The MAK series of kits were not designed for use on chemistry analyzers. There are no applications available for use on chemistry analyzers from Beckman or other manufacturers. The kits, as sold, are intended for manual use. Even if the kits could be adapted to run on the chemistry analyzers, the volumes of reagents supplied are likely insufficient to perform the number of tests advertised for any particular kit.

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  5. It says from the manual that sample absorbance would increase overtime but may I know whether the absorbance of standards (glycerol) will increase overtime as well ?

    1 answer

    1. The glycerol standard is essentially the end product, thus the absorbance will not continue to increase. Lipase in the sample hydrolyzes the triglyceride substrate to form glycerol, which is a process that occurs over time. For this reason, absorbance readings of the samples will continue to increase. The standard curve for this product should be read in end point mode at the end of the incubation time. See the link below to review the kit protocol. Note steps 5 & 6 detail this process.
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/312/996/mak046bul.pdf

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  6. What is the length of the acyl chain of the lipase substrate?

    1 answer

    1. The lipase substrate included in this kit is proprietary. Further information is not available.

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  7. Is possible to use this kit for measuring lipase kit from milk or cheese?

    1 answer

    1. While this kit has not been tested specifically for lipase activity in dairy products, there is no reason to suspect it would not be suitable. Note that some lipases are calcium-dependent which may be relevant to dairy samples. Please review the product information to determine the appropriate sample preparation:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/312/996/mak046bul.pdf

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  8. What kind of material is the POSITIVE CONTROL in KIT?

    1 answer

    1. The positive control is a CV lipase: Chromobacterium viscosum lipase.

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  9. Can these kits be used with serum samples from mouse?

    1 answer

    1. Yes, this kit can be used on mouse serum samples. We generally recommend that fresh serum samples be used. Frozen samples can probably be used. However, the successful use of frozen samples depends on the efficiency of freezing them and the amount of time they have been frozen. If previously frozen serum samples are used, you may want to check the performance of the kit with fresh vs. frozen samples.

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  10. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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