PDB information is not available for this product.
L3126
Lipase from porcine pancreas
Lipase from porcine pancreas
Type II, ≥125 units/mg protein (using olive oil (30 min incubation)), 30-90 units/mg protein (using triacetin)
Synonym(s):
Pancreatic lipase, PPL, Triacylglycerol acylhydrolase, Triacylglycerol lipase
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About This Item
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biological source
Porcine pancreas
Quality Level
type
Type II
form
lyophilized (cake)
specific activity
≥125 units/mg protein (using olive oil (30 min incubation))
30-90 units/mg protein (using triacetin)
composition
protein, ≥20%
storage temp.
2-8°C
InChI
1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
InChI key
QWZUIMCIEOCSJF-CHHCPSLASA-N
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Application
Lipase from porcine pancreas has been used:
Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.
Biochem/physiol Actions
Lipases catalyze the hydrolysis of esters in aqueous solutions and the synthesis of esters in non-aqueous solutions. They also produce hydroxyl and carboxylic groups through the hydrolysis of ester linkages in poly(ethylene terephthalate).
Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.
Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.
Quality
Crude (Steapsin). Contains amylase and protease activity.
Unit Definition
One unit will hydrolyze 1.0 microequivalent of fatty acid from triacetin in 1 hr at pH 7.4 at 37°C. (pH 7.7 is used with olive oil as substrate)
Analysis Note
Protein determined by biuret.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Articles
Lipid Induced Insulin Resistance
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May I know the PDB information related to this lipase protein?
1 answer-
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I want to use this product (L3126) for preparing a solution to conduct in vitro degradation experiments on a particular material. Could you please provide detailed instructions on how to prepare the solution, including any recommendation?
1 answer-
Unfortunately, there is no application specific instructions available for this product. The material may be reconstituted in 100 mM potassium phosphate buffer, pH 8.0, containing 1 mg/ml BSA. This product is from porcine pancreas. Another product option, L3419 Lipase from Thermus thermophilus, is reconstituted in 50 mM Tris-HCl, pH 8.0. This material states that solutions are stable for up to 1 month at 2 - 8°C. Please see the link below to review the datasheet for this item:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/271/504/l3419dat.pdfHelpful?
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How is a "microequivalent" of fatty acid quantified in the enzymatic activity unit definition for L3126?
1 answer-
A microequivalent is equivalent to 1 mmol/L. In this case, one unit of Lipase will hydrolyze 1.0 microequivalent (1 mmol/L) of fatty acid from olive oil in 1 hour at pH 7.7 at 37°C.
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