B8438
Alcian Blue solution
1% in 3% acetic acid, pH 2.5
Synonym(s):
Alcian Blue 8XG solution
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About This Item
UNSPSC Code:
12171500
NACRES:
NA.47
Pricing and availability is not currently available.
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form
liquid
Quality Level
concentration
1% in 3% acetic acid, pH 2.5
technique(s)
microbe id | staining: suitable
color
dark blue to very dark blue
pH range
2.4-2.6
Amax
0.45-0.60 (1:400 in H2O)
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
Application
Alcian Blue solution has been used in the histological staining of acidic glycosaminoglycans (GAGs; mucopolysaccharides) that are carboxylated or sulfated.[1][2] Sulfated glycosaminoglycans appear to be the preferred substrate for alcian blue at pH 2.5.[3] Alcian blue has been used to stain hyaluronan (HA), a nonsulfated GAG, but the intensity of the staining of HA is affected by the method of tissue fixation used.[3] Alcian blue staining of GAGs in tissue may be modified through the addition of an electrolyte such as magnesium chloride.[4]
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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G F Pierce et al.
The American journal of pathology, 138(3), 629-646 (1991-03-01)
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Differential staining of acid glycosaminoglycans (mucopolysaccharides) by alcian blue in salt solutions.
J E Scott et al.
Histochemie. Histochemistry. Histochimie, 5(3), 221-233 (1965-10-01)
W Lin et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 45(8), 1157-1163 (1997-08-01)
The apparent intensity of hyaluronan (HA) staining in tissue sections can vary as a function of fixation techniques. We examined the histochemical distribution of HA in normal human skin using an HA-specific binding peptide derived from bovine nasal cartilage. The
The quantitative determination of glycosaminoglycans in urine with Alcian Blue 8GX.
Whiteman P.
The Biochemical Journal, 131(2), 351-357 (1973)
SPECIFIC STAINING OF SULPHATE GROUPS WITH ALCIAN BLUE AT LOW PH.
R LEV et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 12, 309-309 (1964-04-01)
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