direct ELISA: 1:30,000 dot blot: 1:2,000 immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 western blot (chemiluminescent): 1:160,000
Immunoglobulin is a glycoprotein, with two heavy chain and two light chain connected by disulfide bond. Immunoglobulin g (IgG) is a major class of immunoglobulin. Goat IgG has two subclasses- IgG1 and IgG2. Rabbit anti-Goat IgG is then conjugated to peroxidase by protein cross linking with 0.2% glutaraldehyde. The conjugate is provided as a solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT as a preservative.
Immunogen
Purified goat IgG
Application
Anti-Goat IgG (whole molecule)-Peroxidase antibody produced in rabbit has been used in immunoblotting and immunohistochemistry.
Biochem/physiol Actions
Immunoglobulin mainly helps in immune defense. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reaction.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT.
Preparation Note
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
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Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
The cytoprotective effects of erythropoietin (EPO) and an EPO-related nonerythropoietic analog, pyroglutamate helix B surface peptide (pHBSP), were investigated in an in vitro model of bovine aortic endothelial cell injury under normoxic (21% O
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