IgGs are glycoprotein antibodies that modulate several immune responses. Sheep IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-sheep IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Anti-Sheep IgG (whole molecule)-Peroxidase antibody reacts with all sheep immunoglobulins.
Immunogen
Purified sheep IgG
Application
Anti-Sheep IgG (whole molecule)-Peroxidase antibody is suitable for use in immunoblot.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper)
rElisas and CHEkiT Elisas were performed on serum from Sarda milking ewes using HRP-conjugated donkey anti-sheep IgG at1:1000. The antibody was incubated for 30 minutes at 37 degrees.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin with preservative
Preparation Note
Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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