Skip to Content
Merck
All Photos(2)

Key Documents

View All Documentation

RAB0369

Sigma-Aldrich

Human MMP-7 ELISA Kit

on for cell culture supernatant

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

UNSPSC Code:
41116158
NACRES:
NA.32
Pricing and availability is not currently available.

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 150 pg/mL
standard curve range: 0.137-100 ng/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... MMP7(4316)

Related Categories

General description

The human MMP-7 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MMP-7 in cell culture supernatants. Matrix metalloproteinase-7 (MMP-7)/matrilysin is a small proteolytic enzyme. It secretes zinc- and calcium-dependent endopeptidase. It is expressed in normal endometrial, bronchial, ductal, skin glandular epithelial, urogenital, and gastrointestinal tissues. MMP-7 plays a key role in cell proliferation, migration, invasion, and apoptosis. Elevated expression of MMP-7 is seen in several cancers, such as esophageal, gastric, gallbladder, pancreatic, colorectal, bladder, ovarian, breast, lung, and melanoma.[1]

Immunogen

Recombinant Human MMP7

Application

Human MMP-7 ELISA Kit has been used in ELISA to measure the recovery of the cancer biomarkers/MMP-7 in isothermally vitrified samples.[2] It has also been used in ELISA to determine MMP7 concentrations in multiple myeloma (MM) cell culture supernatants.[3]

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABELADEELISA 5X Assay/Sample Diluent Buffer E (Item E2)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8B - Non-combustible corrosive hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
1129J0222
0615I0222
0422I0222
SAMPLE
1116E0222

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Ruth Eichner et al.
Nature medicine, 22(7), 735-743 (2016-06-14)
Immunomodulatory drugs (IMiDs), such as thalidomide and its derivatives lenalidomide and pomalidomide, are key treatment modalities for hematologic malignancies, particularly multiple myeloma (MM) and del(5q) myelodysplastic syndrome (MDS). Cereblon (CRBN), a substrate receptor of the CRL4 ubiquitin ligase complex, is
Guobao Wang et al.
Arthritis & rheumatology (Hoboken, N.J.), 73(2), 265-275 (2020-09-07)
Flares of lupus nephritis (LN) are frequent and associated with impaired renal prognosis. One major management obstacle in LN flare is the lack of effective methods to identify at-risk patients earlier in their disease course. This study was undertaken to
Hai-Yang Liao et al.
Clinical biochemistry, 92, 9-18 (2021-03-14)
Matrix metalloproteinase-7 (MMP-7) is a small proteolytic enzyme that secretes zinc and calcium endopeptidases. It can degrade a variety of extracellular matrix substrates and other substrates and plays important regulatory roles in many human pathophysiological processes. Since its discovery, MMP-7
Jian Zhang et al.
Kidney & blood pressure research, 42(3), 541-552 (2017-09-19)
In view of the latest findings that matrix metalloproteinase-7 (MMP-7) acted as a vital marker and pathogenic mediator of renal fibrosis in a murine model, we hypothesized that serum MMP-7 level might serve as a noninvasive prognostic biomarker in IgA
Anita M Tuomainen et al.
European journal of preventive cardiology, 21(7), 806-812 (2012-10-20)
Extracellular matrix-degrading proteinases are upregulated in atherosclerotic lesions and can contribute to subsequent pathological events. In the present nested case-control study, we investigated the association of serum concentrations of matrix metalloproteinases MMP-7, MMP-8, and MMP-13, tissue inhibitor of metalloproteinase-1 (TIMP-1)
View All Related Papers

Questions

  1. Is the MMP7 a total form or an active form in this kit?

    1 answer

    1. The capture antibody used in this kit will recognize both the pro and active form of MMP-7. The immunogen is human recombinant MMP-7, aa18 - 267. The standard provided in the kit is the pro form,

      Response from Ray Bio - not to be included in response
      Capture Ab: Mouse Monoclonal IgG2B
      Immunogen: Mouse myeloma cell line NS0-derived recombinant human MMP 7
      aa18-aa267, accession# NP_002414.1
      Specificity: detects human MMP-7 in pro and active forms (seen in WB)

      Detection Ab: Polyclonal Goat IgG
      Immunogen: Mouse myeloma cell line NS0-derived recombinant human MMP 7
      aa18-aa267, accession # P09237

      Additionally, the standard used is recombinant Human MMP-7 in pro form (aa18-267).

      Helpful?

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service