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810255P

Avanti

TopFluor Cholesterol

Avanti Research - A Croda Brand

Synonym(s):

Bodipy Cholesterol; Bdp-Chol

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About This Item

Empirical Formula (Hill Notation):
C36H51BF2N2O
CAS Number:
Molecular Weight:
576.61
UNSPSC Code:
12352211
NACRES:
NA.25

description

23-(dipyrrometheneboron difluoride)-24-norcholesterol

Assay

>99% (TLC)

form

powder

packaging

pkg of 1 × 1 mg (810255P-1mg)
pkg of 1 × 10 mg (810255P-10mg)
pkg of 1 × 5 mg (810255P-5mg)

manufacturer/tradename

Avanti Research - A Croda Brand

shipped in

dry ice

storage temp.

−20°C

General description

Biomolecules attached to BODIPY, is readily absorbed in the visible light. It results in greater fluorescence quantum yields. It is highly photostable and less sensitive to change in polarity and pH.

Application

TOPFLUORCholesterol is suitable:
  • for cholesterol-uptake assay
  • for BODIPY cholesterol labeling in live cell experiments
  • for the formation of giant Janus liposomes via poly(vinyl alcohol) (PVA)-assisted lipid swelling

Biochem/physiol Actions

BODIPY labelled cholesterol has similar properties to that of the naïve cholesterol. This serves as a cholesterol marker for live cells and aids in trafficking intracellular cholesterol. It might also help in indicating lipid storage diseases.

Packaging

5 mL Amber Glass Screw Cap Vial (810255P-10mg)
5 mL Amber Glass Screw Cap Vial (810255P-1mg)
5 mL Amber Glass Screw Cap Vial (810255P-5mg)

Legal Information

Avanti Research is a trademark of Avanti Polar Lipids, LLC
TopFluor is a trademark of Avanti Polar Lipids, LLC

also commonly purchased with this product

Storage Class Code

11 - Combustible Solids

WGK

WGK 3


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Salvatore Chiantia et al.
Biochimica et biophysica acta, 1778(5), 1356-1364 (2008-03-19)
Ceramide-induced alterations in the lateral organization of membrane proteins can be involved in several biological contexts, ranging from apoptosis to viral infections. In order to investigate such alterations in a simple model, we used a combined approach of atomic force
James E Shaw et al.
Biophysical journal, 90(6), 2170-2178 (2005-12-20)
Coupling atomic force microscopy (AFM) with high-resolution fluorescence microscopy is an attractive means of identifying membrane domains by both physical topography and fluorescence. We have used this approach to study the ability of a suite of fluorescent molecules to probe
Silvia Scolari et al.
Biochemistry, 49(42), 9027-9031 (2010-09-25)
Seminal plasma proteins of the fibronectin type II (Fn2) family modulate mammalian spermatogenesis by triggering the release of the lipids phosphatidylcholine and cholesterol from sperm cells. Whereas the specific interaction of these proteins with phosphatidylcholine is well-understood, their selectivity for
Janus Liposomes: Gel-Assisted Formation and Bioaffinity-Directed Clustering
Wang M, et al.
Langmuir, 34(25), 7509-7518 (2018)
Zaiguo Li et al.
The Journal of organic chemistry, 71(4), 1718-1721 (2006-02-14)
Analogues of cholesterol (compounds 1 and 2) and coprostanol (compound 3) containing the BODIPY fluorophore in the aliphatic tail of the free sterol have been synthesized starting with bisnorcholenic acid, cholenic acid 3beta-acetate, and lithocholic acid, respectively. An ester linkage

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