Recommended Products
product name
P1.HTR.TK-, 87121103
biological source
mouse breast
growth mode
Suspension
karyotype
Not specified
morphology
Mastocytoma
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse DBA/2 mastocytoma
Cell Line Description
Derived from the mouse mastocytoma P815 after serial cycles of transfection with a plasmid carrying a TK gene. BUdR is necessary in the growth media to avoid revertants.
Application
Genetic, transfection and tumourigenicity studies
Culture Medium
DMEM + 2mM Glutamine + 100ug/ml Bromo-deoxy Uridine (BUdR) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 8% CO2; 37°C. Media change twice a week.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Certificates of Analysis (COA)
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