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SAB5801029

Sigma-Aldrich

Goat Anti-Mouse IgG2a(Fcγ) Alpaca Heavy chain only Antibody

recombinant Alpaca(VHH) Nano antibody

Synonym(s):

Recombinant Alpaca single variable domain secondary Nanobody

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

alpaca

recombinant

expressed in HEK 293 cells (Human cell line)

conjugate

unconjugated

antibody form

affinity purified immunoglobulin

antibody product type

secondary antibodies

clone

recombinant monoclonal

form

lyophilized solid

mol wt

100 kDa

species reactivity

Alpaca, mouse

technique(s)

ELISA: 1:10000-1:50000
western blot: 1:10000-1:50000

size

0.1 mL

color

colorless

isotype

VHH

shipped in

wet ice

storage temp.

2-8°C

General description

Goat Anti-Mouse IgG2a(Fcγ) Alpaca Heavy chain only Antibody is designed to specifically detect mouse IgG2a Fcγ fragment. It is a monovalent, single domain, recombinant antibody fused to goat IgG Fc. Based on immunoelectrophoresis and/or ELISA, Goat Anti-Mouse IgG2a(Fcγ) Alpaca Heavy chain only Antibody reacts with the Fc fragment of mouse IgG2a selectively but not with other mouse IgG subclasses, mouse IgM, or the Fab portion of mouse immunoglobulins.

Most monoclonal antibodies are generated in mouse. There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from mouse. Each isotype has a different heavy chain. Mouse IgG constitutes 75% of serum immunoglobulins, and IgG is the predominant form of first antibody produced from mouse. Mouse IgG consists of five subclasses-IgG1, IgG2a, IgG2b, IgG2c(inbred mouse strains with the Igh1-b allele have IgG2c isotype instead of IgG2a), IgG3. They are highly homologous and differ mainly in the hinge region. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains, and the heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.

VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(less than15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

Specificity

Mouse IgG2a (Fcγ fragment specific)
Isotype: VHH domain of alpaca IgG2b/2c fused to goat IgG Fc

Immunogen

Recombinant Alpaca single variable domain secondary nano antibody

Packaging

100 μg in Glass bottle

Physical form

Lyophilized from 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose

Reconstitution

Add 100uL dH2O or 100uL dH2O with 50% Glycerol to a final concentration of 1mg/mL

Storage and Stability

After reconstitution, store at –20 °C(Avoid freeze / thaw cycles). Stable for 12 months at -20°C

Other Notes

No cross-reactivity with mouse IgG1/2b/3, mouse IgM, rabbit, human, cynomolgus, rat, goat IgG

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Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317,H412

Hazard Classifications

Aquatic Chronic 3 - Skin Sens. 1

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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