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R6625

Sigma-Aldrich

Ribonucleic acid from torula yeast

Type VI

Synonym(s):

RNA

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25 G
$130.00
100 G
$441.00
1 KG
$2,480.00

$130.00

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All Photos(3)

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25 G
$130.00
100 G
$441.00
1 KG
$2,480.00

About This Item

CAS Number:
63231-63-0
MDL number:
MFCD00132195
UNSPSC Code:
41106305
NACRES:
NA.51

$130.00

In StockDetails

Request a Bulk Order

type

Type VI

Quality Level

200

storage temp.

−20°C

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General description

Ribonucleic Acid (RNA) from torula yeast is the substrate for the RNase enzyme. The isolation of RNA from yeast is a complicated process and involves heating and freezing cycles of cells in the presence of phenyl and detergents (SDS).[1]

Application

Ribonucleic Acid (RNA) is suitable for applications such as northern blot hybridization, reverse transcriptase-polymerase chain reaction (RTPCR), and cDNA construction.[1]

Ribonucleic acid (RNA) from torula yeast may be used as a substrate for studying ribonuclease activities of enzymes such as ribonuclease-A, ribonuclease T1 (RNAase), and bougainvillea xbuttiana antiviral protein 1 (BBAP1)

Quality

Phosphorus: 8.0-10.0% (anhydrous)

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000417297
0000417769
0000417297
0000417769
0000409987

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Questions

1–8 of 8 Questions  

  1. What are the nucleotide sequences of the mixture of RNA? 

    1 answer

    1. This product is a heterogeneous mixture of tRNA molecules This material has not been sequenced.

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  2. How many base pairs are in the RNA? Can I dossolve it in TE buffer?

    1 answer

    1. This product is a heterogeneous mixture of RNA molecules ranging from 3000 to 40000 daltons which corresponds to approximately 10 to 120 base pairs in size. The material is tested for solubility at 10 mg per 1 ml of 0.1M Acetate buffer, pH 5.0. Testing is TE buffer is not performed. However, this buffer is commonly used as short term storage buffer for RNA.

      Please see the link below to review the oligo handling and stability guide for additional information: https://www.sigmaaldrich.com/US/en/technical-documents/protocol/genomics/dna-and-rna-purification/oligonucleotide-handling-and-stability

      Helpful?

  3. I would like to know if this product RNA from torula yeast (R6625) contains only mRNA and rRNA or does it also contains transfer-RNA?

    1 answer

    1. This product is not assayed for the distribution of RNA forms, but it is expected to be a heterogeneous mixture ranging from 3000 to 40000 Daltons.

      Helpful?

  4. What does type VI mean?

    1 answer

    1. The 'type' designation for this product is an internal definition of Sigma and has no specific biological significance. It is in reference to the method of manufacture or product preparation.

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  5. What does Type IV refer to?

    1 answer

    1. The 'type' designation for this product is an internal definition of Sigma and has no specific biological significance. It is in reference to the method of manufacture or product preparation.

      Helpful?

  6. What is the molecular weight of this product? What is the RNA concentration?

    1 answer

    1. This product is a heterogeneous mixture of RNA molecules ranging from 3000 to 40000 in MW. The material is not tested for purity. There is a maximum allowable water content specification of 10%. Please see the link below for review a sample Certificate of Analysis:
      https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/R6625-BULKWXBF1071V.pdf

      Helpful?

  7. What species is Product R6625, Ribonucleic acid from torula yeast, from?

    1 answer

    1. The former taxonomic name for Torula yeast is Torulopsis utilus; the current name is Candida utilus.

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  8. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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