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MAK097

Sigma-Aldrich

Glucose Oxidase Activity Assay Kit

sufficient for 100 colorimetric or fluorometric tests

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric or fluorometric tests

detection method

colorimetric
fluorometric

storage temp.

−20°C

General description

The new Glucose Oxidase Activity Assay Kit, MAK501, is now available! Glucose Oxidase (GOx, EC 1.1.3.4) is an enzyme found in insects, fungi, and bacteria that catalyzes the oxidation of D-glucose to D-gluconolactone. GOx is widely used in the food, beverage, chemical, and pharmaceutical industries.

Suitability

Suitable for the measurement of glucose oxidase (GOx) in a variety of samples including cells and serum

Principle

The Glucose Oxidase Activity Assay Kit provides a simple and direct procedure for measuring GOx activity in a variety of biological samples. GOx activity is determined by a coupled enzyme assay, in which GOx oxidizes D-glucose resulting in the production of hydrogen peroxide (H2O2) that reacts with a probe, generating a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the GOx present. One unit of GOx is defined as the amount of enzyme that generates 1.0 μmole of H2O2 per minute at 37 °C.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Aquatic Chronic 3 - Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup


Certificates of Analysis (COA)

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DNA-guided assembly of a five-component enzyme cascade for enhanced conversion of cellulose to gluconic acid and H2O2.
Chen Q, et al.
Journal of Biotechnology, 263 , 30-35 (2017)
Glucagon-like peptide 1 receptor agonist ameliorates the insulin resistance function of islet β cells via the activation of PDX-1/JAK signaling transduction in C57/BL6 mice with high-fat diet-induced diabetes.
Hao T, et al.
International Journal of Molecular Medicine, 39(4), 1029-1036 (2017)
Umarah Zahoor Banday et al.
Ecotoxicology and environmental safety, 183, 109584-109584 (2019-08-26)
There is a dire need to assess the quality of fishes transported for human consumption as lately, their health is challenged because of anthropogenic activities. Heavy metals with a long environmental persistence are toxic to fishes and the humans. The
Suppression of a single BAHD gene in Setaria viridis causes large, stable decreases in cell wall feruloylation and increases biomass digestibility.
Souza W R, et al.
The New phytologist (2018)
Wagner R de Souza et al.
The New phytologist, 218(1), 81-93 (2018-01-10)
Feruloylation of arabinoxylan (AX) in grass cell walls is a key determinant of recalcitrance to enzyme attack, making it a target for improvement of grass crops, and of interest in grass evolution. Definitive evidence on the genes responsible is lacking

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