Skip to Content
Merck
All Photos(1)

Documents

F6057

Sigma-Aldrich

Fluoroshield™ with DAPI

liquid

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12171500
NACRES:
NA.47

product name

Fluoroshield™ with DAPI, histology mounting medium

Quality Level

200

form

liquid

technique(s)

immunofluorescence: suitable

pH range

7.9-8.3

solubility

water: soluble

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

Related Categories

General description

Fluoroshield with DAPI is an aqueous mounting medium fortified with 4′,6-diamidino-2-phenylindole (DAPI), a nuclear counterstain used as a blue-fluorescent DNA probe. It is an anti-fading agent employed for preserving the fluorescence of tissue and cell smears by preventing rapid photobleaching and minimizing the blinking-mediated phenomena.(1) For use in in situ hybridization techniques or other methods where fluorescence of DNA staining is required. DAPI excites at 360 nm and emits at 460 nm, producing a blue fluorescence. RNA is also stained with DAPI.

Application

Fluoroshield™ with DAPI has been used:
  • as a counterstain in immunocytochemistry to label nuclei of neural progenitor cells
  • as a histology mounting medium for cardiac tissues sections for immunofluorescence studies
  • as mounting solution for immunostained heart sections for nuclei detection

Legal Information

FluoroShield is a trademark of ImmunoBioScience Corp.

related product

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Extracellular matrix-derived extracellular vesicles promote cardiomyocyte growth and electrical activity in engineered cardiac atria
An M, et al.
Biomaterials, 146(1), 49-59 (2017)
Edgar T Hoorntje et al.
PloS one, 13(8), e0203078-e0203078 (2018-08-31)
Likely pathogenic/pathogenic variants in genes encoding desmosomal proteins play an important role in the pathophysiology of arrhythmogenic right ventricular cardiomyopathy (ARVC). However, for a substantial proportion of ARVC patients, the genetic substrate remains unknown. We hypothesized that plectin, a cytolinker
S Hamada et al.
Histochemistry, 79(2), 219-226 (1983-01-01)
DNA-DAPI complexes emit strong bluish white fluorescence when excited by ultraviolet light so that even very small amounts of DNA such as those in mitochondria, chloroplasts, and virus particles can be visualized. Moreover, the staining procedure with DAPI is very
miR-210 suppresses BNIP3 to protect against the apoptosis of neural progenitor cells
Wang F, et al.
Stem Cell Research, 11(1), 657-667 (2013)
Human umbilical cord tissue-derived mesenchymal stromal cells attenuate remodeling after myocardial infarction by proangiogenic, antiapoptotic, and endogenous cell-activation mechanisms
Nascimento DS, et al.
Stem Cell Research & Therapy, 5(1), 5-5 (2014)
View All Related Papers

Articles

Improving Glycoform Coverage for Mucin Prep

Human Protein Atlas uses A-431, U-251 MG, and U-205 cell lines with Prestige Antibodies for organelle mapping.

Fluorescent Live Cell Imaging of LC3 Mediated Autophagy using LentiBrite™ Lentiviral Biosensors

High titer lentiviral particles for LC3 variants used for live cell analysis of cellular autophagy.

Fluorescent Live Cell Imaging of Cytoskeleton Structure Proteins using LentiBrite™ Lentiviral Biosensors

High titer lentiviral particles including beta-actin, alpha-tubulin and vimentin used for live cell analysis of cytoskeleton structure proteins.

3D Organoid Culture: New In Vitro Models of Development and Disease

Organoid culture products to generate tissue and stem cell derived 3D brain, intestinal, gut, lung and cancer tumor organoid models.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service