B6755

BIS-TRIS propane

≥99.0% (titration)

• Synonym(s): 1,3-Bis[tris(hydroxymethyl)methylamino]propane, 2,2′-(Propane-1,3-diylbis(azanediyl))bis(2-(hydroxymethyl)propane-1,3-diol)
• Linear Formula: CH₂[CH₂NHC(CH₂OH)₃]₂
• CAS Number: 64431-96-5
• Molecular Weight: 282.33
• Beilstein: 1786109
• EC Number: 264-899-3
• MDL number: MFCD00004689
• UNSPSC Code: 12161700
• PubChem Substance ID: 24891934
• NACRES: NA.25

Properties

• Quality Level: 400
• Assay: ≥99.0% (titration)
• form: powder
• storage condition: dry at room temperature
• technique(s): HPLC: suitable
• color: white
• useful pH range: 6.3-9.5
• pKa (25 °C): (1) 6.8, (2) 9.0
• mp: 164-165 °C (lit.)
• solubility: water: 30 % (w/w), clear, colorless
• density: 1.179 g/cm³ at 20—25 °C; 1.189 g/cm³ at 20—25 °C
• suitability: suitable for chromatography; suitable for electrophoresis buffers
• application(s): diagnostic assay manufacturing; general analytical; life science and biopharma
• storage temp.: room temp
• SMILES string: OCC(CO)(CO)NCCCNC(CO)(CO)CO
• InChI: 1S/C11H26N2O6/c14-4-10(5-15,6-16)12-2-1-3-13-11(7-17,8-18)9-19/h12-19H,1-9H2
• InChI key: HHKZCCWKTZRCCL-UHFFFAOYSA-N

Description

General description

Bis-Tris Propane buffer, also known as BTP, is a zwitterionic biochemical buffer utilized for pH regulation in various laboratory applications, particularly in molecular biology, biochemistry, and electrophoresis. Its wide buffering range, spanning from pH 6.3 to 9.5, is attributed to its two pKa values of 6.8 (pKa1) and 9.0 (pKa2), which are close. This wide range makes it highly versatile for maintaining stable pH conditions in a variety of experiments.

Furthermore, Bis-Tris Propane is a water-soluble buffer substance that plays a crucial role in enhancing the stability and activity of restriction enzymes. Specifically, its buffering capacity is advantageous for experiments that require pH levels as low as 6-7. When compared to Tris buffer, which has a poor buffering capacity below pH 7.5 and exhibits significant pKa fluctuations with temperature changes, Bis-Tris Propane proves to be a superior choice. Additionally, it finds application in conjunction with hydrochloric acid buffer to stabilize farnesyl diphosphate, particularly when isolated from Saccharomyces cerevisiae strains. Notably, it can also serve as a ligand, forming dinuclear hydroxo complexes with Lanthanides(III).

Application

BIS-TRIS Propane may be used in the following studies:

• as a buffer to investigate the influence of anions with varying nucleophilic properties on the autoxidation of oxymyoglobin (MbO₂).[1]
• for purification of glucose-binding protein from membranes of Sulfolobus solfataricus.[2]
• as a buffer during GeO₂ mineralisation.[3]
• as a buffer component in a study pertaining to environmental research[4]
• as a buffer component to maintain optimal pH conditions during the purification and preparation of engineered enzymes[5]

Features and Benefits

• Suitable for Biological and Biochemical Research
• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
• Effective Buffering from pH 6.3-9.5 (25 °C) with a pKa of 6.8 and 9.0 (25 °C)
• Highly soluble in water

Other Notes

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Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves