The decline in activity over time due to storage conditions has not been determined. Other Proteinase K products may ship at ambient temperature, indicating little or no degradation of the material is expected. However, this items ships on ice. Maintaining the shipping and storage temperature at -20°C is recommended for this product in order to assure optimum performance.
P2308
Proteinase K from Tritirachium album
Proteinase K from Tritirachium album
lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology
Synonym(s):
Endopeptidase K
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5 MG
$89.10
10 MG
$107.00
25 MG
$165.00
100 MG
$472.00
500 MG
$1,650.00
1 G
$2,730.00
$89.10
In StockDetails
Diagnostics Manufacturers should use alternative grade product: SRE0005
Request a Bulk Order
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5 MG
$89.10
10 MG
$107.00
25 MG
$165.00
100 MG
$472.00
500 MG
$1,650.00
1 G
$2,730.00
About This Item
CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54
$89.10
In StockDetails
Diagnostics Manufacturers should use alternative grade product: SRE0005
Request a Bulk Order
Recommended Products
grade
for molecular biology
Quality Level
product line
BioUltra
form
lyophilized powder
specific activity
≥30 units/mg protein
mol wt
28.93 kDa
composition
Protein, ≥90% biuret
impurities
≤1 ppm DNA
foreign activity
DNAse, Nickase and RNAse, none detected
shipped in
wet ice
storage temp.
−20°C
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Related Categories
Application
Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
Determination of enzyme localization on membranes
Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.
Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
Determination of enzyme localization on membranes
Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.
Biochem/physiol Actions
Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.
Unit Definition
One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
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suggested gloves for splash protection
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Articles
Proteinase K aids in molecular biology applications by digesting structural proteins, removing nucleases, and isolating intact genomic DNA.
Pro K aids in disrupting cell membranes for DNA release, crucial for downstream molecular biology techniques.
Guidelines on use of proteinase K, an enzyme commonly used to degrade proteins, and protect DNA and RNA from degradation in samples.
In blood DNA extraction, Proteinase K, an enzyme commonly used to degrade proteins, can help break down the cellular and nuclear membranes, releasing DNA from the cells that protect it from degradation and increase purity/yield making it more suitable for various molecular biology techniques.
Protocols
The GenElute™ Blood Genomic DNA Kit Protocol provides a simple and convenient way to isolate pure genomic DNA from fresh or aged whole blood.
The GenElute Mammalian Genomic DNA Purification Kit Protocol describes the isolation of pure, high molecular weight DNA from a variety of mammalian sources.
Proteinase K activity measured via spectrophotometry using hemoglobin substrate, crucial for enzyme characterization.
GenElute™ Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.
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Hi, I'd like to know what happens if the lyophilized proteinase K (P238) is not stored at -20C for at least a week.
1 answer-
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Is P2308 designed for antigen unmasking during IHC, and is there literature supporting this application?
1 answer-
Proteinase K has not been tested for use with IHC. Product P2308 is specifically tested for use in molecular biology, particularly for preparing cell lysates and releasing nucleic acids, as well as for PCR applications. While the enzyme has been used to digest hair (keratin) and may have other applications, there are no guarantees that P2308 would be suitable for antigen retrieval. No literature references stating that Proteinase K is commonly used for IHC were found.
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Can P2308 be dissolved in water for nucleic acid preparation? If so, at what concentration? Thanks!
1 answer-
This product may be dissolved in water for nucleic acid preparations at 1 mg/mL.
Please see the link below to review the product datasheet which includes preparation instructions:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/116/320/p2308dat.pdfHelpful?
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How to prepare a solution of 20 mg/ml of pK (P2308-5MG)? In the section "Preparation Instructions" it mentions that I need to solve 1 mg in 1 ml of water. Does it means I'll have a final concentration of only 1 mg/ml?
1 answer-
The stock may also be prepared at 20 mg/ml concentration in 50 mM Tris-HCl, pH 8.0, containing 1 mM calcium chloride. This solution is stable for at least 12 months at 4°C. For additional information, please refer to the below publications where the product P2308 has been used.
1) https://www.nature.com/articles/s41598-017-17102-w#Sec9
2) https://www.frontiersin.org/articles/10.3389/fmicb.2018.00830/full#h3
3) https://elifesciences.org/articles/36187#s4Helpful?
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How to prepare Proteinase K solution to use in antigen retrieval of formalin fixed paraffin embedded tissue sections for the purpose of paraffin immunoflorescence? Which is the diluent of choice to prepare the solution from powder form of proteinase K.
1 answer-
This product is not tested for use in antigen retrieval. The product 21627, IHC Select Proteinase K protocol may be applied to this product, P2308. To prepare a stock solution, dissolve Proteinase K, P2308, at 0.2mg/mL in 0.05M Tris solution, pH 7.5.
For antigen retrieval in FFPA tissue sections (deparaffinized) the recommended dilution of the stock solution is 1:30.
End users would need to optimize final dilutionHelpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the recommended pH range for Product P2308, Proteinase K?
1 answer-
Proteinase K is normally used in the pH range of 7.5-8.0.
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How should I store solutions of Product P2308, Proteinase K?
1 answer-
Stock solutions in 40% glycerol of at least 20 mg/ml can be stored in 10 mM Tris-HCl buffer, pH 7.5.with 1 mM calcium acetate (see product P4850). Solutions are also stable at pH 8 at 4°C.
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What amount of Product P2308, Proteinase K, should I use for nucleic acid preparations?
1 answer-
Amounts for typical usage is 50-200 μg/mL.
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How should I prepare solutions of Product P2308, Proteinase K?
1 answer-
Solutions can be prepared in 25-50 mM Tris-HCl buffer, pH 8.0, containing 1-5 mM Calcium chloride.
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