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storage temp.
2-8°C
suitability
mycobacteria
General description
Middlebrook ADC growth supplement is used to isolate a wide variety of Mycobacteria including M. tuberculosis. It contains Bovine albumin, dextrose, and catalase. Bovine albumin neutralizes the toxic products. Dextrose is the fermentable sugar and catalase cleaves the toxic hydrogen peroxides which creates stress to the Mycobacteria cells thus, making it an ideal media for the cultivation of Mycobacterial species
Application
Specialized culture media supplement, designed to isolate and cultivate various Mycobacterial species.
Components
Ingredients: (per vial) Bovine albumin fraction V 2.5g Dextrose 1.0g Catalase 0.0015g Distilled water 50 ml
Reconstitution
Warm up the refrigerated supplement to 45-50°C. Aseptically add the contents of the vial to 450 ml of sterile Middlebrook 7H9 Broth Base (M0178). Mix well and pour into sterile screw capped tubes.
BMC complementary and alternative medicine, 17(1), 128-128 (2017-02-27)
Tuberculosis is an infectious communicable disease and the causative agent of the disease has over the years developed resistance to streamline chemotherapeutic agents with dire consequences and there is a need for development of new and more potent alternatives. Constituents
Cell wall hydrolases are enzymes that cleave bacterial cell walls by hydrolyzing specific bonds within peptidoglycan and other portions of the envelope. Two major sources of hydrolases in nature are from hosts and microbes. This study specifically investigated whether cell
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