PC213L
Anti-GABA Rabbit pAb
lyophilized, Calbiochem®
Synonym(s):
Anti-Gamma Amino-Butyric Acid
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352203
NACRES:
NA.43
Recommended Products
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized
does not contain
preservative
species reactivity
rat
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
isotype
IgG
shipped in
ambient
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
rat ... Gabra1(29705)
General description
Anti-GABA, rabbit polyclonal, recognizes GABA in rat thalamus and cerebellum. It is validated for use in immunohistochemistry on frozen sections.
Rabbit polyclonal antibody supplied as lyophilized undiluted serum. Recognizes GABA.
Recognizes GABA in rat thalamus and cerebellum.
Immunogen
GABA
Rat
Application
Frozen Sections (1:12,000-1:15,000, ABC detection or 1:3000-1:5000, PAP detection; see comments)
Warning
Toxicity: Standard Handling (A)
Physical form
Undiluted serum.
Reconstitution
Reconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. For storage, dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
Analysis Note
Positive Control
Rat thalamus or cerebellum
Rat thalamus or cerebellum
Other Notes
Antibody specificity was examined in the rat thalamus and cerebellum. Glutaraldehyde is a necessary component of fixation for use with this antibody. Higher concentrations of glutaraldehyde (1%-2%) may be used as needed. The specificity of the antibody for GABA was evaluated using a competitive inhibition ELISA. A GABA conjugate control completely eliminated labeling, while 1000-fold excess of the following conjugates: alanine, aspartate, beta alanine, glutamate, glycine, taurine, and tyrosine did not inhibit the binding to the GABA conjugate control. Antibody should be titrated for optimal results in individual systems.
Sudhof, T.C. 1995. Nature375, 645.
Hokfelt, T., et al. 1987. Experientia Suppl.56, 154.
Hokfelt, T., et al. 1987. Experientia Suppl.56, 154.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Not finding the right product?
Try our Product Selector Tool.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Todd McLaughlin et al.
Investigative ophthalmology & visual science, 64(14), 40-40 (2023-11-28)
Diabetic retinopathy (DR) is a leading cause of blindness in working-age adults characterized by retinal dysfunction and neurovascular degeneration. We previously reported that deletion of X-box binding protein 1 (XBP1) leads to accelerated retinal neurodegeneration in diabetes; however, the mechanisms
Abram Akopian et al.
The Journal of comparative neurology, 527(1), 159-173 (2016-07-14)
We determined whether the structural and functional integrity of amacrine cells (ACs), the largest cohort of neurons in the mammalian retina, are affected in glaucoma. Intraocular injection of microbeads was made in mouse eyes to elevate intraocular pressure as a
Abram Akopian et al.
The Journal of clinical investigation, 127(7), 2647-2661 (2017-06-13)
The progressive death of retinal ganglion cells and resulting visual deficits are hallmarks of glaucoma, but the underlying mechanisms remain unclear. In many neurodegenerative diseases, cell death induced by primary insult is followed by a wave of secondary loss. Gap
Todd McLaughlin et al.
Cells, 12(12) (2023-06-28)
p58IPK is a multifaceted endoplasmic reticulum (ER) chaperone and a regulator of eIF2α kinases involved in a wide range of cellular processes including protein synthesis, ER stress response, and macrophage-mediated inflammation. Systemic deletion of p58IPK leads to age-related loss of
Zheng-Li Fan et al.
PloS one, 14(12), e0227200-e0227200 (2020-01-01)
Schizophrenia (SZ) is a serious and incurable mental disorder characterized by clinical manifestations of positive and negative symptoms and cognitive dysfunction. High-frequency deep brain stimulation (DBS) of the ventral hippocampus (VHP) has been recently applied as a therapeutic approach for
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service