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GLP1T-36HK

Sigma-Aldrich

Glucagon Like Peptide-1 (Total) RIA

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84

grade

for protein analysis

Quality Level

100

description

ED<sub>80</sub> = 14 ± 3 pM ED<sub>50</sub> = 53 ± 6 pM ED<sub>20</sub> = 224 ± 20 pM
analytes available: Glucagon Like Peptide-1
 10-1000 pg/mL (standard curve range)

feature

accuracy 79–119%

species reactivity

human, mouse, rat

technique(s)

radioimmunoassay: suitable

UniProt accession no.

P01275

detection method

radioactive

General description

FUNCTION: SwissProt: P01275 # Glicentin may modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life.
SIZE: 180 amino acids; 20909 Da
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP1 and GLP2 are also secreted in selected neurons in the brain.
PTM: Proglucagon is post-translationally processed in a tissue- specific manner in pancreatic A cells and intestinal L cells. In pancreatic A cells, the major bioactive hormone is glucagon cleaved by PCSK2/PC2. In the intestinal L cells PCSK1/PC1 liberates GLP-1, GLP-2, glicentin and oxyntomodulin. GLP-1 is further N-terminally truncated by post-translational processing in the intestinal L cells resulting in GLP-1(7-37) GLP-1-(7-36)amide. The C-terminal amidation is neither important for the metabolism of GLP-1 nor for its effects on the endocrine pancreas.
SIMILARITY: SwissProt: P01275 ## Belongs to the glucagon family.
MISCELLANEOUS: In the glucagon antagonist, His-53 and Phe-58 are missing. This antagonist has been successfully utilized to reduce glucose concentration in vivo."

Application

Two-day disequilibrium assay at 4°C. Sample volume = 300 μL plasma (extraction required)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Skull and crossbonesEnvironment
GHS06,GHS09

Signal Word

Danger

Hazard Statements

H302 + H332,H311,H411

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Certificates of Analysis (COA)

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